Analytical and Bioanalytical Chemistry

, Volume 400, Issue 10, pp 3409–3417 | Cite as

Determination of steroid hormones in blood by GC–MS/MS

  • Martin Hansen
  • Naja W. Jacobsen
  • Frederik K. Nielsen
  • Erland Björklund
  • Bjarne Styrishave
  • Bent Halling-Sørensen
Original Paper

Abstract

This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08–0.16 ng/mL for estrogens, 0.20–0.36 ng/mL for androgens and 0.36–0.43 ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50–112% in the range 0.10 to 2.00 ng/mL.

Keywords

Plasma Serum Solid-phase extraction Gas chromatography Lipid separation Biological samples 

Supplementary material

216_2011_5038_MOESM1_ESM.pdf (338 kb)
ESM 1(PDF 337 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Martin Hansen
    • 1
  • Naja W. Jacobsen
    • 1
  • Frederik K. Nielsen
    • 1
  • Erland Björklund
    • 1
  • Bjarne Styrishave
    • 1
  • Bent Halling-Sørensen
    • 1
  1. 1.Section of Toxicology and Environmental Chemistry, Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical SciencesUniversity of CopenhagenCopenhagenDenmark

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