Analytical and Bioanalytical Chemistry

, Volume 400, Issue 10, pp 3395–3407 | Cite as

Kinetic analyses and performance of a colloidal magnetic nanoparticle based immunoassay dedicated to allergy diagnosis

  • Bruno Teste
  • Frédéric Kanoufi
  • Stéphanie Descroix
  • Pascal Poncet
  • Thomas Georgelin
  • Jean-Michel Siaugue
  • Jan Petr
  • Anne Varenne
  • Marie-Claire Hennion
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

In this paper, we demonstrate the possibility to use magnetic nanoparticles as immunosupports for allergy diagnosis. Most immunoassays used for immunosupports and clinical diagnosis are based on a heterogeneous solid-phase system and suffer from mass-transfer limitation. The nanoparticles’ colloidal behavior and magnetic properties bring the advantages of homogeneous immunoassay, i.e., species diffusion, and of heterogeneous immunoassay, i.e., easy separation of the immunocomplex and free forms, as well as analyte preconcentration. We thus developed a colloidal, non-competitive, indirect immunoassay using magnetic core–shell nanoparticles (MCSNP) as immunosupports. The feasibility of such an immunoassay was first demonstrated with a model antibody and described by comparing the immunocapture kinetics using macro (standard microtiter plate), micro (microparticles) and nanosupports (MCSNP). The influence of the nanosupport properties (surface chemistry, antigen density) and of the medium (ionic strength, counter ion nature) on the immunocapture efficiency and specificity was then investigated. The performances of this original MCSNP-based immunoassay were compared with a gold standard enzyme-linked immunosorbent assay (ELISA) using a microtiter plate. The capture rate of target IgG was accelerated 200-fold and a tenfold lower limit of detection was achieved. Finally, the MCSNP-based immunoassay was successfully applied to the detection of specific IgE from milk-allergic patient’s sera with a lower LOD and a good agreement (CV < 6%) with the microtiter plate, confirming the great potential of this analytical platform in the field of immunodiagnosis.

Keywords

Allergy diagnosis IgE Colloidal immunoassay Kinetic reaction Magnetic core–shell nanoparticles 
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Notes

Acknowledgment

This work was supported by the French research agency ANR SOLUDIAG ANR-07-BLAN-02660.

Supplementary material

216_2011_5021_MOESM1_ESM.pdf (837 kb)
ESM 1 (PDF 836 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Bruno Teste
    • 1
  • Frédéric Kanoufi
    • 1
  • Stéphanie Descroix
    • 1
  • Pascal Poncet
    • 1
    • 4
  • Thomas Georgelin
    • 2
  • Jean-Michel Siaugue
    • 2
  • Jan Petr
    • 3
  • Anne Varenne
    • 3
  • Marie-Claire Hennion
    • 1
  1. 1.Physicochimie des Electrolytes, Colloïdes et Sciences Analytiques (PECSA) UMR 7195 CNRS-ESPCI-UPMCParis Cedex 05France
  2. 2.CNRS-UPMC-ESPCIParis Cedex 05France
  3. 3.CNRS-ENSCP-UPMC, Ecole Nationale Supérieure de Chimie de Paris, Chimie Paris TechParis Cedex 05France
  4. 4.Institut PasteurParisFrance

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