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Analytical and Bioanalytical Chemistry

, Volume 400, Issue 7, pp 2085–2091 | Cite as

The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme

  • Xinyi Wang
  • Yao Xu
  • Yang Chen
  • Limei Li
  • Feng Liu
  • Na LiEmail author
Original Paper

Abstract

We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.

Figure

Schematic description and demonstration of aggregation of DNA aptamer stabalized AuNPs for colorimetric and PRLS sensing of lysozyme.

Keywords

Gold nanoparticles Aggregation Lysozyme DNA aptamer Plasmon resonance light scattering Visual detection 

Notes

Acknowledgment

This work was supported by the National Natural Science Foundation of China (nos. 20975004 and 21035005) and Instrumental Analysis Fund of Peking University.

Supplementary material

216_2011_4943_MOESM1_ESM.pdf (17 kb)
(PDF 17 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xinyi Wang
    • 1
    • 2
  • Yao Xu
    • 1
  • Yang Chen
    • 1
  • Limei Li
    • 2
  • Feng Liu
    • 1
  • Na Li
    • 1
    Email author
  1. 1.Beijing National Laboratory for Molecular Sciences (BNLMS), The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina
  2. 2.College of SciencesShenyang Agricultural UniversityShenyangChina

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