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Analytical and Bioanalytical Chemistry

, Volume 401, Issue 1, pp 89–101 | Cite as

Distribution of lipids in human brain

  • Antonio Veloso
  • Roberto Fernández
  • Egoitz Astigarraga
  • Gabriel Barreda-Gómez
  • Iván Manuel
  • M. Teresa Giralt
  • Isidro Ferrer
  • Begoña Ochoa
  • Rafael Rodríguez-Puertas
  • José A. Fernández
Original Paper

Abstract

The enormous abundance of lipid molecules in the central nervous system (CNS) suggests that their role is not limited to be structural and energetic components of cells. Over the last decades, some lipids in the CNS have been identified as intracellular signalers, while others are known to act as neuromodulators of neurotransmission through binding to specific receptors. Neurotransmitters of lipidic nature, currently known as neurolipids, are synthesized during the metabolism of phospholipid precursors present in cell membranes. Therefore, the anatomical identification of each of the different lipid species in human CNS by imaging mass spectrometry (IMS), in association with other biochemical techniques with spatial resolution, can increase our knowledge on the precise metabolic routes that synthesize these neurolipids and their localization. The present study shows the lipid distribution obtained by MALDI-TOF IMS in human frontal cortex, hippocampus, and striatal area, together with functional autoradiography of cannabinoid and LPA receptors. The combined application of these methods to postmortem human brain samples may be envisioned as critical to further understand neurological diseases, in general, and particularly, the neurodegeneration that accompanies Alzheimer’s disease.

Keywords

Cannabinoid Lysophosphatidic acid MALDI-TOF Mercaptobenzothiazole Lipidomics Imaging mass spectrometry 

Abbreviations

2-AG

2-Arachidonylglycerol

AEA

Anandamide

CB

Cannabinoids

CNS

Central nervous system

eCB

Endocannabinoids

GPCR

G-protein coupled receptor

IMS

Imaging mass spectrometry

LPA

Lysophosphatidic acid

MALDI

Matrix-assisted laser desorption/ionization

MBT

2-Mercaptobenzothiazole

MS

Mass spectrometry

PC

Phosphatidylcholines

PC(O)

Plasmalogen

PCA

Principal component analysis

PE

Phosphatidylethanolamines

PG

Phosphatidylglycerols

PI

Phosphatidylinositols

PLSA

Probabilistic latent semantic analysis

PS

Phosphatidylserines

PUFA

Polyunsaturated fatty acids

SF

Sulfatides

SM

Sphingomyelin

TOF

Time of flight

Notes

Acknowledgements

This study was supported by the Spanish Ministry of Education and Science (SAF2007-60211), the Basque Government (IT-325-07, IT-336-10, IT-440-10, S-PE10UN50 and SAI07/46) and Carlos III Health Institute (FIS PI070628). A. V. is recipient of a UPV/EHU graduate fellowship.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Antonio Veloso
    • 1
  • Roberto Fernández
    • 1
  • Egoitz Astigarraga
    • 1
  • Gabriel Barreda-Gómez
    • 2
  • Iván Manuel
    • 2
  • M. Teresa Giralt
    • 2
  • Isidro Ferrer
    • 4
  • Begoña Ochoa
    • 3
  • Rafael Rodríguez-Puertas
    • 2
  • José A. Fernández
    • 1
  1. 1.Department of Chemical Physics, Faculty of Science and TechnologyUniversity of the Basque CountryLeioaSpain
  2. 2.Department of PharmacologyUniversity of the Basque CountryLeioaSpain
  3. 3.Department of Physiology; Faculty of Medicine and DentistryUniversity of the Basque CountryLeioaSpain
  4. 4.Institute of NeuropathologyIDIBELL, University Hospital Bellvitge, Hospitalet de LlobregatBarcelonaSpain

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