Analytical and Bioanalytical Chemistry

, Volume 400, Issue 9, pp 2755–2761 | Cite as

Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)

  • Anne März
  • Bettina Mönch
  • Petra Rösch
  • Michael Kiehntopf
  • Thomas Henkel
  • Jürgen Popp
Original Paper


In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.


SERS Microfluidic lab-on-a-chip Enzyme activity Thiopurine methyltransferase 6-Mercaptopurine 



We gratefully acknowledge the Free State of Thuringia and the European Union (EFRE) for financial support under support code 2008FE9112 and 2008FE9113 (BioOptiSens).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Anne März
    • 1
  • Bettina Mönch
    • 2
  • Petra Rösch
    • 1
  • Michael Kiehntopf
    • 2
  • Thomas Henkel
    • 3
  • Jürgen Popp
    • 1
    • 3
  1. 1.Institute of Physical ChemistyFriedrich Schiller UniversityJenaGermany
  2. 2.Department of Clinical Chemistry and Laboratory DiagnosticsUniversity Hospital JenaJenaGermany
  3. 3.Institute of Photonic Technology (IPHT)JenaGermany

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