Analytical and Bioanalytical Chemistry

, Volume 400, Issue 2, pp 459–468 | Cite as

Hexylacrylate-based mixed-mode monolith, a stationary phase for the nano-HPLC separation of structurally related enkephalins

  • Ayat Abbood
  • Christine Herrenknecht
  • Gaëlle Proczek
  • Stéphanie Descroix
  • Jordi Rodrigo
  • Myriam Taverna
  • Claire SmadjaEmail author
Original Paper


The potential of an in situ photopolymerized hexylacrylate-based monolithic stationary phase-bearing sulfonic acid groups was investigated by studying the chromatographic retention of small structurally related peptides (enkephalins) by nano-LC. Several retention mechanisms were highlighted. First, a reverse-phase chromatographic behavior toward neutral solutes due to hexylacrylate-moieties was demonstrated. Second, an evaluation of the influences of buffer pH suggested the involvement of a cation-exchange mechanism due to the presence of 2-acrylamido-2-methyl-1-propanesulfonic acid. This cation-exchange phenomenon was confirmed by the clear influence of Na+ concentration in the mobile phase on peptide retention.


Monolith Enkephalin Peptides Chromatography Retention Cation-exchange 



We thank Tichrine-University of Syria for providing Ayat Abbood’s scholarship. This work was supported in part by the FW6 Neurotas project.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ayat Abbood
    • 1
  • Christine Herrenknecht
    • 2
  • Gaëlle Proczek
    • 3
  • Stéphanie Descroix
    • 3
  • Jordi Rodrigo
    • 4
  • Myriam Taverna
    • 1
  • Claire Smadja
    • 1
    Email author
  1. 1.Univ. Paris-Sud, UMR CNRS 8612, LPNSS, Faculté de PharmacieChâtenay-MalabryFrance
  2. 2.Univ. Nantes, EA2160, Mer Molécules Santé (MMS) Faculté de PharmacieNantesFrance
  3. 3.ESPCI, Laboratoire Environnement et Chimie Analytique, UMR CNRS 7121Paris Cedex 05France
  4. 4.Univ. Paris-Sud-BioCIS, UMR CNRS 8076, Faculté de PharmacieChâtenay-MalabryFrance

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