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Analytical and Bioanalytical Chemistry

, Volume 400, Issue 2, pp 527–534 | Cite as

Simultaneous determination of weakly ionizable analytes in urine and plasma samples by transient pseudo-isotachophoresis in capillary zone electrophoresis

  • Igor Botello
  • Francesc Borrull
  • Marta Calull
  • Carme AguilarEmail author
Original Paper

Abstract

A rapid method for the simultaneous determination of several non-steroidal anti-inflammatory drugs (NSAIDs) in human plasma and urine was developed using transient pseudo-isotachophoresis (ITP) in capillary zone electrophoresis (CZE). The influence of different parameters on resolution and preconcentration efficiency, such as background electrolyte (BGE) composition, sample injection, sample matrix composition, and pH, were studied to optimize the transient pseudo-ITP performance. Optimized conditions were a BGE consisting of 100 mM Na2B4O7 in 10% aqueous MeOH solution and hydrodynamic injection of the sample at 50 mbar for 90 s. The sample was prepared in a solution mixture of 1% NaCl/ethanol (30:70 v/v) at pH 10. Our results show that this simple strategy offers improved sensitivity compared to conventional CZE analysis, reaching a 45-fold preconcentration factor. The detection limits (LODs) were as low as 0.07 mg/L for standard samples with good repeatability (values of relative standard deviation, %RSD < 11%). The method was applied to the analysis of NSAIDs in biological samples. Validation for human plasma and urine samples demonstrated good linearity, low detection limits, and satisfactory repeatability values.

Keywords

Capillary electrophoresis Non-steroidal anti-inflammatory drugs Plasma samples Transient pseudo-isotachophoresis Urine samples 

Abbreviations

BGE

background electrolyte

CE

capillary electrophoresis

CZE

capillary zone electrophoresis

ITP

isotachophoresis

NSAID

non-steroidal anti-inflammatory drug

SEF

sensitivity enhancement factor

Transient pseudo-ITP

transient pseudo-isotachophoresis

Notes

Acknowledgment

This study was funded by the General Research Directorate of the Spanish Ministry of Science and Technology, projects CTM2008-06847-C02-01/TECNO and CTM2008-0825 and by the Generalitat de Catalunya, Department d’Innovació, Universitats i Empreses, project 2009 SGR 223.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Igor Botello
    • 1
  • Francesc Borrull
    • 1
  • Marta Calull
    • 1
  • Carme Aguilar
    • 1
    Email author
  1. 1.Departament de Química Analítica i Química Orgànica, Facultat de QuímicaUniversitat Rovira i VirgiliTarragonaSpain

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