Advertisement

Analytical and Bioanalytical Chemistry

, Volume 400, Issue 2, pp 547–560 | Cite as

Structural and biological evaluation of a multifunctional SWCNT-AgNPs-DNA/PVA bio-nanofilm

  • Ramesh P. Subbiah
  • Haisung Lee
  • Murugan Veerapandian
  • Sathya Sadhasivam
  • Soo-won Seo
  • Kyusik YunEmail author
Original Paper

Abstract

A bio-nanofilm consisting of a tetrad nanomaterial (nanotubes, nanoparticles, DNA, polymer) was fabricated utilizing in situ reduction and noncovalent interactions and it displayed effective antibacterial activity and biocompatibility. This bio-nanofilm was composed of homogenous silver nanoparticles (AgNPs) coated on single-walled carbon nanotubes (SWCNTs), which were later hybridized with DNA and stabilized in poly(vinyl alcohol) (PVA) in the presence of a surfactant with the aid of ultrasonication. Electron microscopy and bio-AFM (atomic force microscopy) images were used to assess the morphology of the nanocomposite (NC) structure. Functionalization and fabrication were examined using FT–Raman spectroscopy by analyzing the functional changes in the bio-nanofilm before and after fabrication. UV–visible spectroscopy and X-ray powder diffraction (XRD) confirmed that AgNPs were present in the final NC on the basis of its surface plasmon resonance (370 nm) and crystal planes. Thermal gravimetric analysis was used to measure the percentage weight loss of SWCNT (17.5%) and final SWCNT-AgNPs-DNA/PVA (47.7%). The antimicrobial efficiency of the bio-nanofilm was evaluated against major pathogenic organisms. Bactericidal ratios, zone of inhibition, and minimum inhibitory concentration were examined against gram positive and gram negative bacteria. A preliminary cytotoxicity analysis was conducted using A549 lung cancer cells and IMR-90 fibroblast cells. Confocal laser microscopy, bio-AFM, and field emission scanning electron microscopy (FE-SEM) images demonstrated that the NCs were successfully taken up by the cells. These combined results indicate that this bio-nanofilm was biocompatible and displayed antimicrobial activity. Thus, this novel bio-nanofilm holds great promise for use as a multifunctional tool in burn therapy, tissue engineering, and other biomedical applications.

 

Schematic representation of the fabrication of bio-nanofilm, and it's photographic images and applications.

Keywords

Hybrid Characterization Antibacterial Cytotoxicity Cellular uptake Skin film 

Notes

Acknowledgements

This work was supported by Kyungwon University research fund in 2010 and Grant No. 10032112 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy. This study was supported by a grant of the Ministry of Health & Welfare (A040041) and Samsung Biomedical Research Institute, Republic of Korea (PB00021).

Supplementary material

216_2011_4757_MOESM1_ESM.pdf (612 kb)
ESM 1 (PDF 612 kb)

References

  1. 1.
    Mertens DM, Jenkins ME, Warden GD (1997) Outpatient burn management. Nurs Clin North Am 32:343–364Google Scholar
  2. 2.
    Gang RK, Bang RL, Sanyal SC, Mokaddas E, Lari AR (1999) Pseudomonas aeruginosa septicaemia in burns. Burns 25:611–616CrossRefGoogle Scholar
  3. 3.
    Cook N (1998) Methicillin-resistant Staphylococcus aureus versus the burn patient. Burns 24:91–98CrossRefGoogle Scholar
  4. 4.
    Potokar T, Shoba C, Ali S (1998) International network for training, education and research in burns. Indian J Plast Surg 40:107CrossRefGoogle Scholar
  5. 5.
    Reig A, Tejerina C, Codina J, Mirabet V (1992) Infection in burn patients. Ann Mediterr Burns Club 5:91–95Google Scholar
  6. 6.
    Mahar P, Padiglione AA, Cleland H, Paul E, Hinrichs M, Wasiak J (2010) Pseudomonas aeruginosa bacteraemia in burns patients: risk factors and outcomes. Burns 36:1228–1233CrossRefGoogle Scholar
  7. 7.
    Hench LL, Jones JR (2005) Biomaterials, artificial organs and tissue engineering. Woodhead, CambridgeCrossRefGoogle Scholar
  8. 8.
    Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83CrossRefGoogle Scholar
  9. 9.
    Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145:83–96CrossRefGoogle Scholar
  10. 10.
    Fong J, Wood F, Fowler BA (2005) Silver coated dressing reduces the incidence of early burn wound cellulitis and associated costs of inpatient treatment: comparative patient care audits. Burns 31:562–567CrossRefGoogle Scholar
  11. 11.
    Lee HY, Park HK, Lee YM, Kim K, Park SB (2007) A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications. Chem Commun 28:2959–2961CrossRefGoogle Scholar
  12. 12.
    Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranbabu S, Vaidyanathan R, Eom SH (2009) Antiangiogenic properties of silver nanoparticles. Biomaterials 30:6341–6350CrossRefGoogle Scholar
  13. 13.
    Krutyakov YA, Kudrinskiy AA, Olenin AY, Lisichkin GV (2008) Synthesis and properties of silver nanoparticles: advances and prospects. Chem Rev 77:228–233Google Scholar
  14. 14.
    Ajayan PM, Schadler LS, Giannaris C, Rubio A (2000) Single-walled carbon nanotube–polymer composites: strength and weakness. Adv Mater 12:750–753CrossRefGoogle Scholar
  15. 15.
    Shin SR, Lee CK, So I, Jeon J, Kang TM, Kee CW, Kim SI, Spinks GM, Wallace GG, Kim SJ (2008) DNA-wrapped single-walled carbon nanotube hybrid fibers for supercapacitors and artificial muscles. Adv Mater 20:466–470CrossRefGoogle Scholar
  16. 16.
    Subbiah R, Veerapandian M, Yun KS (2010) Nanoparticles: functionalization and multifunctional applications in biomedical sciences. Curr Med Chem 17:4559–4577CrossRefGoogle Scholar
  17. 17.
    Bandyopadhyay A, Sarkar MD, Bhowmic AK (2005) Poly(vinyl alcohol)/silica hybrid nanocomposites by sol-gel technique: synthesis and properties. J Mater Sci 40:5233–5241CrossRefGoogle Scholar
  18. 18.
    Rangari VK, Mohammad GM, Jeelani S, Hundley A, Vig K, Singh SR, Pillai S (2010) Synthesis of Ag/CNT hybrid nanoparticles and fabrication of their Nylon-6 polymer nanocomposite fibers for antimicrobial applications. Nanotechnology 21:095102–095113CrossRefGoogle Scholar
  19. 19.
    Burczak K, Gamian E, Kochman A (1996) Long-term in vivo performance and biocompatibility of poly(vinyl alcohol) hydrogel macrocapsules for hybrid-type artificial pancreas. Biomaterials 17:2351–2356CrossRefGoogle Scholar
  20. 20.
    Paul W, Sharma CP (1997) Acetylsalicylic acid loaded poly(vinyl alcohol) hemodialysis membranes: effect of drug release on blood compatibility and permeability. J Biomater Sci Polym Ed 8:755–764CrossRefGoogle Scholar
  21. 21.
    Kobayashi M (2004) A study of polyvinyl alcohol-hydrogel (PVA-H) artificial meniscus in vivo. Biomed Mater Eng 14:505–515Google Scholar
  22. 22.
    Zheng Y, Huang X, Wang Y, Xu H, Chen X (2009) Performance and characterization of irradiated poly(vinyl alcohol)/polyvinyl pyrrolidone composite hydrogels used as cartilages replacement. J Appl Polym Sci 113:736–741CrossRefGoogle Scholar
  23. 23.
    Ma Y, Zheng Y, Huang X, Xi T, Lin X, Han D, Song W (2010) Mineralization behavior and interface properties of BG-PVA/bone composite implants in simulated body fluid. Biomed Mater 5:025003–025011CrossRefGoogle Scholar
  24. 24.
    Millon LE, Guhados G, Wan W (2008) Anisotropic polyvinyl alcohol-bacterial cellulose nanocomposite for biomedical applications. J Biomed Mater Res B Appl Biomater 86B:444–452CrossRefGoogle Scholar
  25. 25.
    Parikh DV, Fink T, Rajasekharan K, Sachinvala ND, Sawhney APS, Calamari TA (2005) Antimicrobial silver/sodium carboxymethyl cotton dressings for burn wounds. Text Res J 75:134–138CrossRefGoogle Scholar
  26. 26.
    Jeon HJ, Yi SC, Oh SG (2003) Preparation and antibacterial effects of Ag-SiO2 thin films by sol-gel method. Biomaterials 24:4921–4928CrossRefGoogle Scholar
  27. 27.
    Bryaskova R, Pencheva D, Kale GM, Lad U, Kandardjiev T (2010) Synthesis, characterization and antibacterial activity of PVA/TEOS/Ag-Np hybrid thin films. J Colloid Interface Sci 349:77–85CrossRefGoogle Scholar
  28. 28.
    Xing Z, Chae W, Baek J, Choi M, Jung Y, Kang I (2010) In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering. Biomacromolecules 11:1248–1253CrossRefGoogle Scholar
  29. 29.
    Amsterdam D (1996) Susceptibility testing of antimicrobials in liquid media. Williams and Wilkins, BaltimoreGoogle Scholar
  30. 30.
    Paiva MC, Zhou B, Fernando KAS, Lin Y, Kennedy JM, Sun Y-P (2004) Mechanical and morphological characterization of polymer-carbon nanocomposites from functionalized carbon nanotubes. Carbon 42:2849–2854CrossRefGoogle Scholar
  31. 31.
    Jorio A, Souza Filho AG, Dresselhaus G, Dresselhaus MS, Swan AK, Goldberg BB, Pimenta MA, Hafner JH, Lieber CM, Saito R (2002) G-band resonant Raman study of 62 isolated single-wall carbon nanotubes. Phys Rev B 65:155412–155421CrossRefGoogle Scholar
  32. 32.
    Duguid J, Bloomfield VA, Benevides J, Thomas GJ (1993) Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd. Biophys J 65:1916–1928CrossRefGoogle Scholar
  33. 33.
    Thomas J, Wang AHJ (1988) Laser Raman spectroscopy of nucleic acids. Nucleic acids mol Biol 2:1–30Google Scholar
  34. 34.
    Benevides JM, Stow PL, Ilag LL, Incardona NL, Thomas GJ (1991) Crystal and solution structures of the B-DNA dodecamer d(CGCAAATTTGCG) probed by Raman spectroscopy: heterogeneity in the crystal structure does not persist in the solution structure. Biochemistry 30:4855–4863CrossRefGoogle Scholar
  35. 35.
    Thomas PS, Stuart BH (1997) A Fourier transform Raman spectroscopy study of water sorption by poly(vinyl alcohol). Spectrochim Acta A 53:2275–2278CrossRefGoogle Scholar
  36. 36.
    Kataura H, Kumazawa Y, Maniwa Y, Umezu I, Suzuki S, Ohtsuka Y, Achiba Y (1999) Optical properties of single-wall carbon nanotubes. Synth Met 103:2555–2558CrossRefGoogle Scholar
  37. 37.
    O’Connell MJ, Bachilo SM, Huffman CB, Moore VC, Strano MS, Haroz EH, Rialon KL, Boul PJ, Noon WH, Kittrell C, Ma J, Hauge RH, Weisman RB, Smalley RE (2002) Band gap fluorescence from individual single-walled carbon nanotubes. Science 297:593–596CrossRefGoogle Scholar
  38. 38.
    Buzaneva E, Karlash A, Yakovkin K, Shtogun Y, Putselyk S, Zherebetskiy D, Gorchinskiy A, Popova G, Prilutska S, Matyshevska O, Prilutskyy Y, Lytvyn P, Scharff P, Eklund P (2002) DNA nanotechnology of carbon nanotube cells: physico-chemical models of self-organization and properties. Mater Sci Eng C 19:41–45CrossRefGoogle Scholar
  39. 39.
    Rather SU, Naik M, Hwang SW, Kim AR, Nahm KS (2009) Room temperature hydrogen uptake of carbon nanotubes promoted by silver metal catalyst. J Alloys Compd 475:L17–L21CrossRefGoogle Scholar
  40. 40.
    Ram S, Gautam A, Fecht HJ, Cai J, Bansmann H, Behm RJ (2007) A new allotropic structure of silver nanocrystals nucleated and grown over planar polymer molecules. Philos Mag Lett 87:361–372CrossRefGoogle Scholar
  41. 41.
    Chang JH, Kim SJ, Im S (2004) Poly(trimethylene terephthalate) nanocomposite fibers by in situ intercalation polymerization: thermo-mechanical properties and morphology (I). Polymer 45:5171–5181CrossRefGoogle Scholar
  42. 42.
    Veerapandian M, Lim SK, Nam HM, Kuppannan G, Yun KS (2010) Glucosamin-functionalized silver glyconanoparticles: characterization and antibacterial activity. Anal Bioanal Chem 398:867–876CrossRefGoogle Scholar
  43. 43.
    Butkus MA, Edling L, Labare MPJ (2003) The efficacy of silver as a bactericidal agent: advantages, limitations and considerations for future use. Water Supply Res Technol AQUA 52:407–416Google Scholar
  44. 44.
    Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52:662–668CrossRefGoogle Scholar
  45. 45.
    Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353CrossRefGoogle Scholar
  46. 46.
    Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model. J Colloid Interface Sci 275:177–182CrossRefGoogle Scholar
  47. 47.
    Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM (2008) Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224. J Mater Sci Technol 24:192–196Google Scholar
  48. 48.
    Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park CY, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH (2007) Antimicrobial effects of silver nanoparticles. Nanomedicine 3:95–101Google Scholar
  49. 49.
    Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–983CrossRefGoogle Scholar
  50. 50.
    Navarro E, Baun A, Behra R, Hartmann NB, Filser J, Miao AJ, Quigg A, Santschi PH, Sigg L (2008) Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi. Ecotoxicology 17:372–386CrossRefGoogle Scholar
  51. 51.
    Sur I, Cam D, Kahraman M, Baysal A, Culha M (2010) Interaction of multi-functional silver nanoparticles with living cells. Nanotechnology 21:175104–175114CrossRefGoogle Scholar
  52. 52.
    Shvedova AA, Kagan VE (2010) The role of nanotoxicology in realizing the ‘helping without harm’ paradigm of nanomedicine: lessons from studies of pulmonary effects of single-walled carbon nanotubes. J Intern Med 267:106–118CrossRefGoogle Scholar
  53. 53.
    Alt V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R (2004) “Plugging into enzymes”: nanowiring of redox enzymes by a gold nanoparticle. Biomaterials 25:4383–4391CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ramesh P. Subbiah
    • 1
  • Haisung Lee
    • 2
  • Murugan Veerapandian
    • 1
  • Sathya Sadhasivam
    • 1
  • Soo-won Seo
    • 2
  • Kyusik Yun
    • 1
    Email author
  1. 1.College of BionanotechnologyKyungwon UniversityGyeonggi-doSouth Korea
  2. 2.Interdisciplinary Graduate Program of Biomedical Engineering, School of MedicineSungkyunkwan UniversitySeoulKorea

Personalised recommendations