Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins
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A new approach is reported on the use of poly(N-isopropylacrylamide) (PNIPAM)-coated molecularly imprinted beads (coated MIP beads) for controlling the release of protein. The coated MIP beads were composed of double layers, an internal thermosensitive lysozyme-imprinted layer, and an external PNIPAM layer. The coated MIP beads were prepared by two-step surface-initiated living-radical polymerization (SIP). In this systemic study, the coated MIP beads had good selectivity to the template protein (lysozyme) and temperature stimulus-responsive behavior, both of which were superior to those of MIP beads having a layer of thermosensitive lysozyme-imprinted polymer only. Using the coated MIP beads, reference proteins and the template lysozyme could be released separately at 38 °C and at 23 °C. The corresponding coated non-imprinted beads (coated NIP beads) did not have such double thermosensitive “gates” with specific selectivity for a particular protein. The proposed smart controlled imprinted system for protein is attractive for chemical carriers, drug-delivery system, and sensors.
KeywordsThermosensitive Molecularly imprinted bead Protein
We would like to thank the National Basic Research Program of China (973 Program, nos. 2007CB914100 and 2011CB707703) and the National Nature Science Foundation of China (nos. 20875049 and 21075069).
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