Analytical and Bioanalytical Chemistry

, Volume 400, Issue 8, pp 2565–2576 | Cite as

Identification and quantification of flavonoids and ellagic acid derivatives in therapeutically important Drosera species by LC–DAD, LC–NMR, NMR, and LC–MS

  • Martin Zehl
  • Christina Braunberger
  • Jürgen Conrad
  • Marija Crnogorac
  • Stanimira Krasteva
  • Bernhard Vogler
  • Uwe Beifuss
  • Liselotte Krenn
Original Paper

Abstract

Droserae herba is a drug commonly used for treatment of convulsive or whooping cough since the seventeenth century. Because of the contribution of flavonoids and ellagic acid derivatives to the therapeutic activity of Droserae herba, an LC–DAD method has been developed for quantification of these analytes in four Drosera species used in medicine (Drosera anglica, D. intermedia, D. madagascariensis, and D. rotundifolia). During elaboration of the method 13 compounds, including three substances not previously described for Drosera species, were detected and unambiguously identified by means of extensive LC–MS and LC–NMR experiments and by off-line heteronuclear 2D NMR after targeted isolation. The most prominent component of D. rotundifolia and D. anglica, 2″-O-galloylhyperoside, with myricetin-3-O-β-glucopyranoside and kaempferol-3-O-(2″-O-galloyl)-β-galactopyranoside, were identified for the very first time in this genus. The LC–DAD method for quantification was thoroughly validated, and enables, for the first time, separation and precise analysis of these analytes in Droserae herba. Simple sample preparation and use of a narrow-bore column guarantee low cost and simplicity of the suggested system, which is excellently suited to quality control of the drug or herbal medicinal products containing this drug.

Figure

2″-O-galloylhyperoside - a major compound in Drosera anglica and Drosera rotundifolia

Keywords

Drosera Identification Quantification LC–DAD LC–MS LC–NMR 

Supplementary material

216_2011_4690_MOESM1_ESM.pdf (395 kb)
ESM 1(PDF 394 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Martin Zehl
    • 1
  • Christina Braunberger
    • 2
  • Jürgen Conrad
    • 2
  • Marija Crnogorac
    • 1
  • Stanimira Krasteva
    • 1
  • Bernhard Vogler
    • 3
  • Uwe Beifuss
    • 2
  • Liselotte Krenn
    • 1
  1. 1.Department of PharmacognosyUniversity of ViennaViennaAustria
  2. 2.Institute of ChemistryHohenheim UniversityStuttgartGermany
  3. 3.Department of ChemistryUniversity of Alabama in HuntsvilleHuntsvilleUSA

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