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Analytical and Bioanalytical Chemistry

, Volume 400, Issue 1, pp 25–31 | Cite as

Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine

  • Takeshi SaitoEmail author
  • Tomokazu Fukushima
  • Yuko Yui
  • Shota Miyazaki
  • Akihiro Nakamoto
  • Akira Namera
  • Sadaki Inokuchi
Original Paper

Abstract

We present a method based on monolitic spin column extraction and gas chromatography–mass spectrometry as an analytical method for screening diquat (DQ), paraquat (PQ), and fenitrothion in serum and urine. This method is useful for clinical and forensic toxicological analyses. Recovery of DQ, PQ, and fenitrothion from serum and urine, spiked at concentrations between 0.1, 2.5, 20, and 45 μg/ml, ranged from 51.3% to 106.1%. Relative standard deviation percentages were between 3.3% and 14.8%. Detection and quantitation limits for serum and urine were 0.025 and 0.05 μg/ml, respectively, for DQ, 0.1 and 0.1 μg/ml, respectively, for PQ, and 0.025 and 0.05 μg/ml, respectively, for fenitrothion. Therefore, these compounds can be detected and quantified in the case of acute poisoning.

Keywords

Diquat Paraquat Fenitrothion Monolitic spin column extraction GC-MS 

Notes

Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (C) (No. 21590745) from the Japan Society for the Promotion of Science.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Takeshi Saito
    • 1
    Email author
  • Tomokazu Fukushima
    • 1
  • Yuko Yui
    • 2
  • Shota Miyazaki
    • 2
  • Akihiro Nakamoto
    • 3
    • 4
  • Akira Namera
    • 4
  • Sadaki Inokuchi
    • 1
  1. 1.Department of Emergency and Critical Care MedicineTokai University School of MedicineIseharaJapan
  2. 2.GL Sciences IncIrumaJapan
  3. 3.Scientific Investigation LaboratoryHiroshima Prefectural Police HeadquartersHiroshimaJapan
  4. 4.Department of Forensic Medicine, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan

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