Analytical and Bioanalytical Chemistry

, Volume 399, Issue 3, pp 1011–1020 | Cite as

Characterisation of workplace aerosols in the manganese alloy production industry by electron microscopy

  • Kjersti Gjønnes
  • Asbjørn Skogstad
  • Siri Hetland
  • Dag G. Ellingsen
  • Yngvar ThomassenEmail author
  • Stephan Weinbruch
Paper in Forefront


Workplace aerosols in a combined FeMn and SiMn alloy smelter were studied by scanning and transmission electron microscopy. Special emphasis was placed on the characterisation of individual particles with diameters below 500 nm and on identification of the different manganese phases present in the workroom air. In high-carbon FeMn production, the submicron size fraction is dominated by MnO particles forming chain-like or compact agglomerates. Minor amounts of MnO2, Mn3O4, Mn2O3 and Fe3O4 are also observed. During production of SiMn, the submicron size fraction consists predominantly of MnSi particles, but small amounts of Mn3Si, Mn6Si and Mn5Si2 are also found. Workplace aerosols from the manganese oxide refinement (MOR) process consist mostly of Mn oxides. Minor amounts of carbonaceous particles occurring as sheets, ribbons and as hollow carbon structures are observed along the whole production line. Carbonaceous particles are either amorphous or consist of poorly crystallised graphite. Particles with fibre morphology were encountered at all sampling locations but most prominently during tapping of FeMn with fibre concentrations between 0.1 and 0.7 per cm3. The pronounced differences in particle composition along the production line clearly show that workers are exposed to a variety of Mn-containing species. MnO particles have a higher solubility than MnSi particles and are thus more bioaccessible, suggesting a higher risk of adverse health effects in the FeMn production than in the SiMn production.


TEM bright field image shows chain-like agglomerates of SiMn primary nanoparticles found in workroom air during casting of SiMn alloy


Workplace Aerosol Manganese Electron microscopy Speciation 



TEM investigations were carried out at the Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, Ås, and at the Centre for Materials Science at the University of Oslo.


  1. 1.
    Couper J (1837) On the effects of black oxide of manganese when inhaled into the lungs. Br Ann Med Pharmacol 1:41–42Google Scholar
  2. 2.
    McMillan DE (1999) A brief history of the neurobehavioral toxicity of manganese: some unanswered questions. Neurotoxicology 20:499–507Google Scholar
  3. 3.
    Rodier J (1955) Manganese poisoning in Moroccan miners. Br J Ind Med 12:21–35Google Scholar
  4. 4.
    Reidies AH (1990) Manganese compounds. In: Elvers B, Hawkins S, Schulz G (eds) Ullmann’s encyclopedia of industrial chemistry, vol A16, 5th edn. VCH Verlagsgesellschaft, WeinheimGoogle Scholar
  5. 5.
    Gunst S, Weinbruch S, Wentzel M, Ortner HM, Skogstad A, Hetland S, Thomassen Y (2000) Chemical composition of individual aerosol particles in workplace air during production of manganese alloys. J Environ Monit 2:65–71CrossRefGoogle Scholar
  6. 6.
    Thomassen Y, Ellingsen DG, Hetland S, Sand G (2001) Chemical speciation and sequential extraction of Mn in workroom aerosols: analytical methodology and results from a field study in Mn alloy plants. J Environ Monit 3:555–559CrossRefGoogle Scholar
  7. 7.
    Ellingsen DG, Hetland SM, Thomassen Y (2003) Manganese air exposure assessment and biological monitoring in the manganese alloy production industry. J Environ Monit 5:84–90CrossRefGoogle Scholar
  8. 8.
    Breysse PN (1991) Electron microscopic analysis of airborne asbestos fibres. Crit Rev Anal Chem 22:201–227CrossRefGoogle Scholar
  9. 9.
    Yamate G, Agarwal SC and Gibbons RD (1984) Methodology for the measurement of airborne asbestos by electron microscopy. Report 698-02-3266, Environment Protection Agency, Washington DCGoogle Scholar
  10. 10.
    World Health Organization (1997) Determination of airborne fibre number concentrations. World Health Organization, GenevaGoogle Scholar
  11. 11.
    Weinbruch S, van Aken P, Ebert M, Thomassen Y, Skogstad A, Chashchin VP, Nikonov A (2002) The heterogeneous composition of working place aerosols in a nickel refinery: a transmission and scanning electron microscope study. J Environ Monit 4:344–350CrossRefGoogle Scholar
  12. 12.
    Höflich BLW, Weinbruch S, Theissmann R, Gorzawski H, Ebert M, Ortner HM, Skogstad A, Ellingsen DG, Drabløs PA, Thomassen Y (2005) Characterization of individual aerosol particles in workroom air of aluminium smelter potrooms. J Environ Monit 7:419–424CrossRefGoogle Scholar
  13. 13.
    Farrants G, Schüler B, Karlsen J, Reith A, Langård S (1989) Characterization of the morphological properties of welding fume particles by transmission electron microscopy and digital image analysis. Am Ind Hyg Assoc J 50:473–479CrossRefGoogle Scholar
  14. 14.
    Jenkins NT, Eagar TW (2005) Chemical analysis of welding fume particles. Weld J 84:s87–s93Google Scholar
  15. 15.
    Köylü ÜO, Faeth GM, Farias TL, Carvalho MG (1995) Fractal and projected structure properties of soot aggregates. Combust Flame 100:621–633CrossRefGoogle Scholar
  16. 16.
    Köylü ÜO, Xing Y, Rosner DE (1995) Fractal morphology analysis of combustion-generated aggregates using angular scattering and electron microscope images. Langmuir 11:4848–4854CrossRefGoogle Scholar
  17. 17.
    Wentzel M, Gorzawski H, Naumann KH, Saathoff H, Weinbruch S (2003) Transmission electron microscopical and aerosol dynamical characterization of soot aerosols. J Aerosol Sci 34:1347–1370CrossRefGoogle Scholar
  18. 18.
    Wellbeloved DB, Craven PM, Wandby JW (1990) Manganese and manganese alloys. In: Elvers B, Hawkins S, Schulz G (eds) Ullmann’s encyclopedia of industrial chemistry, vol A16, 5th edn. VCH Verlagsgesellschaft, WeinheimGoogle Scholar
  19. 19.
    Roels H, Meiers G, Delos M, Ortega I, Lauwerys R, Buchet JP, Lison D (1997) Influence of the route of administration and the chemical form (MnCl2, MnO2) on the absorption and cerebral distribution of manganese in rats. Arch Toxicol 71:223–230CrossRefGoogle Scholar
  20. 20.
    Dorman DC, Struve MF, Arden James R, Marshall MW, Parkinson CU, Wong BA (2001) Influence of particle solubility on the delivery of inhaled manganese to the rat brain: manganese sulfate and manganese tetroxide pharmacokinetics following repeated (14-day) exposure. Toxicol Appl Pharmacol 170:79–87CrossRefGoogle Scholar
  21. 21.
    Inoue K, Takano H, Yanagisawa R, Sakurai M, Ichinose T, Sadakane K, Yoshikawa T (2005) Effects of nano particles on antigen-related airway inflammation in mice. Respir Res 6:106CrossRefGoogle Scholar
  22. 22.
    Inoue K, Takano H, Yanagisawa R, Ichinose T, Sakurai M, Yoshikawa T (2006) Effects of nano particles on cytokine expression in murine lung in the absence or presence of allergen. Arch Toxicol 80:614–619CrossRefGoogle Scholar
  23. 23.
    Pulskamp K, Diabaté S, Krug HF (2007) Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. Toxicol Lett 168:58–74CrossRefGoogle Scholar
  24. 24.
    Nygaard UC, Hansen JS, Samuelsen M, Alberg T, Marioara CD, Løvik M (2009) Single-walled and multiple-walled carbon nanotubes promote allergic immune responses in mice. Toxicol Sci 109:113–123CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Kjersti Gjønnes
    • 1
  • Asbjørn Skogstad
    • 1
  • Siri Hetland
    • 1
  • Dag G. Ellingsen
    • 1
  • Yngvar Thomassen
    • 1
    • 2
    Email author
  • Stephan Weinbruch
    • 3
  1. 1.National Institute of Occupational HealthOsloNorway
  2. 2.Department of Plant and Environmental SciencesNorwegian University of Life SciencesÅsNorway
  3. 3.Institute of Applied GeosciencesTechnical University of DarmstadtDarmstadtGermany

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