Analytical and Bioanalytical Chemistry

, Volume 399, Issue 1, pp 519–524

Integrated microfluidic device for the separation and electrochemical detection of catechol estrogen-derived DNA adducts

  • Abdulilah Dawoud Bani-Yaseen
  • Toshikazu Kawaguchi
  • Alexander K. Price
  • Christopher T. Culbertson
  • Ryszard Jankowiak
Technical Note

Abstract

Catechol estrogen-derived DNA adducts are formed as a result of the reaction of catechol estrogen metabolites (e.g., catechol estrogen quinones) with DNA to form depurinating adducts. Developing a new methodology for the detection of various DNA adducts is essential for medical diagnostics, and to this end, we demonstrate the applicability of on-chip capillary electrophoresis with an integrated electrochemical system for the separation and amperometric detection of various catechol estrogen-derived DNA adducts. A hybrid PDMS/glass microchip with in-channel amperometric detection interfaced with in situ palladium decoupler is utilized and presented. The influence of buffer additives along with the effect of the separation voltage on the resolving power of the microchip is discussed. Calibration plots were constructed in the range 0.4–10 μM with r2 ≥ 0.999, and detection limits in the attomole range are reported. These results suggest that on-chip analysis is applicable for analyzing various DNA adducts as potential biomarkers for future medical diagnostics.

Keywords

Microfluidic device Catechol estrogens Amperometric detection DNA adduct Separation Integration 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Abdulilah Dawoud Bani-Yaseen
    • 1
    • 2
  • Toshikazu Kawaguchi
    • 3
  • Alexander K. Price
    • 1
  • Christopher T. Culbertson
    • 1
  • Ryszard Jankowiak
    • 1
  1. 1.Department of ChemistryKansas State UniversityManhattanUSA
  2. 2.Department of ChemistryTaibah UniversityAl-Madinah Al-MunawarahSaudi Arabia
  3. 3.Arts, Science, and Technology Center for Cooperative ResearchKyushu UniversityKasugaJapan

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