Analytical and Bioanalytical Chemistry

, Volume 399, Issue 3, pp 1295–1304 | Cite as

Extraction and determination of chloramphenicol in feed water, milk, and honey samples using an ionic liquid/sodium citrate aqueous two-phase system coupled with high-performance liquid chromatography

  • Juan Han
  • Yun Wang
  • Cui-lan Yu
  • Yong-sheng Yan
  • Xue-qiao Xie
Original Paper


A green, simple, non-toxic, and sensitive sample pretreatment procedure coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of chloramphenicol (CAP) that exploits an aqueous two-phase system based on imidazolium ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim]BF4) and organic salt (Na3C6H5O7) using a liquid–liquid extraction technique. The influence factors on partition behaviors of CAP were studied, including the type and amount of salts, the pH value, the volume of [Bmim]BF4, and the extraction temperature. Extraction efficiency of the CAP was found to increase with increasing temperature and the volume of [Bmim]BF4. Thermodynamic studies indicated that hydrophobic interactions were the main driving force, although electrostatic interactions and salting-out effects were also important for the transfer of the CAP. Under the optimal conditions, 90.1% of the CAP could be extracted into the ionic liquid-rich phase in a single-step extraction. This method was practical when applied to the analysis of CAP in feed water, milk, and honey samples with a linear range of 2~1,000 ng mL−1. The method yielded a limit of detection of 0.3 ng mL−1 and a limit of quantification of 1.0 ng mL−1. The recovery of CAP was 90.4–102.7% from aqueous samples of real feed water, milk, and honey samples by the proposed method. This novel process is much simpler and more environmentally friendly and is suggested to have important applications for the separation of antibiotics.


The schematic diagram of separation target molecule in ILATPS


Ionic liquid extraction Aqueous two-phase system Chloramphenicol Food High-performance liquid chromatography 



This work has been supported by the National Natural Science Foundation of China (no. 20777029, no. 20976074, and no. 21076098), the Natural Science Foundation of Jiangsu Province (no. BK2010349), and the Ph.D. Programs Foundation of Ministry of Education of China (no. 200807100004)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Juan Han
    • 1
  • Yun Wang
    • 1
  • Cui-lan Yu
    • 1
  • Yong-sheng Yan
    • 1
  • Xue-qiao Xie
    • 1
  1. 1.School of Environment, School of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiangJiangsuChina

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