Analytical and Bioanalytical Chemistry

, Volume 399, Issue 1, pp 429–439

Quantification of isotope-labelled and unlabelled folates in plasma, ileostomy and food samples

  • Barbara E. Büttner
  • Veronica E. Öhrvik
  • Cornelia M. Witthöft
  • Michael Rychlik
Original Paper

DOI: 10.1007/s00216-010-4313-4

Cite this article as:
Büttner, B.E., Öhrvik, V.E., Witthöft, C.M. et al. Anal Bioanal Chem (2011) 399: 429. doi:10.1007/s00216-010-4313-4

Abstract

New stable isotope dilution assays were developed for the simultaneous quantitation of [13C5]-labelled and unlabelled 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, folic acid along with unlabelled tetrahydrofolic acid and 10-formylfolic acid in clinical samples deriving from human bioavailability studies, i.e. plasma, ileostomy samples, and food. The methods were based on clean-up by strong anion exchange followed by LC-MS/MS detection. Deuterated analogues of the folates were applied as the internal standards in the stable isotope dilution assays. Assay sensitivity was sufficient to detect all relevant folates in the respective samples as their limits of detection were below 0.62 nmol/L in plasma and below 0.73 μg/100 g in food or ileostomy samples. Quantification of the [13C5]-label in clinical samples offers the possibility to differentiate between folate from endogenous body pools and the administered dose when executing bioavailability trials.

Figure

Differentiation of folate isotopologues by tandem mass spectrometry

Keywords

Folate Ileostomal effluent Plasma LC-MS/MS Stable isotope dilution assay 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Barbara E. Büttner
    • 1
  • Veronica E. Öhrvik
    • 3
  • Cornelia M. Witthöft
    • 3
  • Michael Rychlik
    • 1
    • 2
  1. 1.Division BIOANALYTIK Weihenstephan, Research Center for Nutrition and Food Sciences (Z I E L)Technische Universität MünchenFreising-WeihenstephanGermany
  2. 2.Analytical Food ChemistryTechnische Universität MünchenFreisingGermany
  3. 3.Dept. of Food ScienceSwedish University of Agricultural SciencesUppsalaSweden

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