Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene
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This paper describes the application of plasmonics-based nanoprobes that combine the modulation of the plasmonics effect to change the surface-enhanced Raman scattering (SERS) of a Raman label and the specificity of a DNA hairpin loop sequence to recognize and discriminate a variety of molecular target sequences. Hybridization with target DNA opens the hairpin and physically separates the Raman label from the metal nanoparticle thus reducing the plasmonics effect and quenching the SERS signal of the label. We have successfully demonstrated the specificity and selectivity of the nanoprobes in the detection of a single-nucleotide polymorphism (SNP) in the breast cancer BRCA1 gene in a homogenous solution at room temperature. In addition, the potential application of plasmonics nanoprobes for quantitative DNA diagnostic testing is discussed.
KeywordsPlasmonics Surface-enhanced Raman scattering Single-nucleotide polymorphism Breast cancer
This research was partly sponsored by the National Institutes of Health (Project # R01 EB006201), and by the US Department of Energy, Office of Health and Environmental Research under contract No. DEAC0500OR22725 with UT-Battelle, L.L.C. M. B. Wabuyele was also supported by an appointment to the Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.