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Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 227–238 | Cite as

Cell-based assays: fuelling drug discovery

  • Elisa Michelini
  • Luca Cevenini
  • Laura Mezzanotte
  • Andrea Coppa
  • Aldo RodaEmail author
Review

Abstract

It has been estimated that over a billion dollars in resources can be consumed to obtain clinical approval, and only a few new chemical entities are approved by the US Food and Drug Administration (FDA) each year. Therefore it is of utmost importance to obtain the maximum amount of information about biological activity, toxicological profile, biochemical mechanisms, and off-target interactions of drug-candidate leads in the earliest stages of drug discovery. Cell-based assays, because of their peculiar advantages of predictability, possibility of automation, multiplexing, and miniaturization, seem the most appealing tool for the high demands of the early stages of the drug-discovery process. Nevertheless, cellular screening, relying on different strategies ranging from reporter gene technology to protein fragment complementation assays, still presents a variety of challenges. This review focuses on main advantages and limitations of different cell-based approaches, and future directions and trends in this fascinating field.

Figure

Different cell-based strategies can improve the throughput and reliability of the first stages of the drug discovery process

Keywords

Drug monitoring/drug screening Cell systems/single-cell analysis Biosensors Biochips/high-throughput screening Bioanalytical methods 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Elisa Michelini
    • 1
    • 2
  • Luca Cevenini
    • 1
  • Laura Mezzanotte
    • 1
  • Andrea Coppa
    • 1
  • Aldo Roda
    • 1
    • 2
    Email author
  1. 1.Department of Pharmaceutical SciencesUniversity of BolognaBolognaItaly
  2. 2.INBB, Istituto Nazionale di Biostrutture e BiosistemiBolognaItaly

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