Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 489–498 | Cite as

Ultra-fast two-dimensional microchip electrophoresis using SDS μ-CGE and microemulsion electrokinetic chromatography for protein separations

  • John K. Osiri
  • Hamed Shadpour
  • Steven A. Soper
Original Paper


A poly(methyl methacrylate) microfluidic chip was used to perform a two-dimensional (2-D) separation of a complex protein mixture in short development times. The separation was performed by combining sodium dodecyl sulfate micro-capillary gel electrophoresis (SDS μ-CGE) with microemulsion electrokinetic chromatography (μ-MEEKC), which were used for the first and second dimensions, respectively. Fluorescently labeled Escherichia coli cytosolic proteins were profiled by this 2-D approach with the results compared to a similar 2-D separation using SDS μ-CGE × μ-MEKC (micelle electrokinetic chromatography). The relatively short column lengths (effective length = 10 mm) for both dimensions were used to achieve separations requiring only 220 s of development time. High spot production rates (131 ± 11 spots min−1) and reasonable peak capacities (481 ± 18) were generated despite the fact that short columns were used. In addition, the use of μ-MEEKC in the second dimension was found to produce higher peak capacities compared to μ-MEKC (481 ± 18 for μ-MEEKC and 332 ± 17 for μ-MEKC) due to the higher plate numbers associated with μ-MEEKC.


Two-dimensional electrophoresis Protein profiling E. coli proteins SDS micro-CGE micro-MEEKC PMMA microchip 



The authors would like to express their gratitude for financial support of this work by the Louisiana Board of Regents and the National Science Foundation (EPS-0346411). The authors would also like to thank the World Class University (WCU) program for partial financial support of this work.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • John K. Osiri
    • 1
    • 2
    • 5
  • Hamed Shadpour
    • 1
    • 2
    • 6
  • Steven A. Soper
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of ChemistryLouisiana State UniversityBaton RougeUSA
  2. 2.Center for BioModular Multi-Scale SystemsLouisiana State UniversityBaton RougeUSA
  3. 3.Department of Mechanical EngineeringLouisiana State UniversityBaton RougeUSA
  4. 4.Nano-Biotechnology and Chemical EngineeringUlsan National Institute of Science and TechnologyUlsanSouth Korea
  5. 5.Department of Chemical EngineeringWashington State UniversityPullmanUSA
  6. 6.Department of ChemistryUniversity of North CarolinaChapel HillUSA

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