Analytical and Bioanalytical Chemistry

, Volume 397, Issue 5, pp 1883–1892

Optimization and single-laboratory validation study of a high-performance liquid chromatography (HPLC) method for the determination of phenolic Echinacea constituents

Original Paper

DOI: 10.1007/s00216-010-3763-z

Cite this article as:
Brown, P.N., Chan, M. & Betz, J.M. Anal Bioanal Chem (2010) 397: 1883. doi:10.1007/s00216-010-3763-z


Three species of Echinacea (Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida) are commonly used for medicinal purposes. The phenolic compounds caftaric acid, cichoric acid, echinacoside, cynarin, and chlorogenic acid are among the phytochemical constituents that may be responsible for the purported beneficial effects of the herb. Although methods for the analysis for these compounds have been published, documentation of their validity was inadequate as the accuracy and precision for the detection and quantification of these phenolics was not systematically determined and/or reported. To address this issue, the high-performance liquid chromatography method, originally developed by the Institute for Nutraceutical Advancement (INA), was reviewed, optimized, and validated for the detection and quantification of these phenolic compounds in Echinacea roots and aerial parts.


Echinacea Phenolic Chromatography Method validation Quality control 

Copyright information

© Crown copyright in right of Canada 2010

Authors and Affiliations

  • Paula N. Brown
    • 1
  • Michael Chan
    • 1
  • Joseph M. Betz
    • 2
  1. 1.British Columbia Institute of TechnologyBurnabyCanada
  2. 2.Office of Dietary SupplementsNational Institutes of HealthBethesdaUSA

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