Analytical and Bioanalytical Chemistry

, Volume 397, Issue 4, pp 1457–1466 | Cite as

Optical recognition of salivary proteins by use of molecularly imprinted poly(ethylene-co-vinyl alcohol)/quantum dot composite nanoparticles

  • Mei-Hwa Lee
  • Yun-Chao Chen
  • Min-Hsien Ho
  • Hung-Yin Lin
Original Paper

Abstract

Molecularly imprinted polymers (MIPs) have long been studied for applications in biomolecule recognition and binding; compared with natural antibodies, they may offer advantages in cost and stability. We report on the development of MIPs that “self-report” concentrations of bound analytes via fluorescence changes in embedded quantum dots (QDots). Composite QDot/MIPs were prepared using phase inversion of poly(ethylene-co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of salivary target molecules (e.g. amylase, lipase, and lysozyme). These major protein components of saliva have been implicated as possible biomarkers for pancreatic cancer. The optimum (highest imprinting effectiveness) ethylene mole ratios of the commercially available EVALs were found to be 32, 38, and 44 mol% for the imprinting of amylase, lipase, and lysozyme, respectively. QD fluorescence quenching was observed on binding of analytes to composite MIPs in a concentration-dependent manner, and was used to construct calibration curves. Finally, the composite MIP particles were used for the quantitative detection of amylase, lipase, and lysozyme in real samples (saliva) and compared with a commercial Architect ci 8200 chemical analysis system.

Keywords

Molecular imprinting Quantum dots Salivary proteins Poly(ethylene-co-ethylene alcohol) Composite particles 

Notes

Acknowledgements

We appreciate financial support from National Science Council of ROC under Contract No. NSC 98-2220-E-390-002.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mei-Hwa Lee
    • 1
  • Yun-Chao Chen
    • 2
  • Min-Hsien Ho
    • 2
  • Hung-Yin Lin
    • 2
    • 3
  1. 1.Department of Materials Science and EngineeringI-Shou UniversityKaohsiungTaiwan
  2. 2.Department of Chemical and Materials EngineeringNational University of KaohsiungKaohsiungTaiwan
  3. 3.Department of Chemical and Materials EngineeringNational University of Kaohsiung (NUK)KaohsiungTaiwan

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