Hydrogen exchange mass spectrometry: what is it and what can it tell us?
- 1.1k Downloads
Proteins are undoubtedly some of the most essential molecules of life. While much is known about many proteins, some aspects still remain mysterious. One particularly important aspect of understanding proteins is determining how structure helps dictate function. Continued development and implementation of biophysical techniques that provide information about protein conformation and dynamics is essential. In this review, we discuss hydrogen exchange mass spectrometry and how this method can be used to learn about protein conformation and dynamics. The basic concepts of the method are described, the workflow illustrated, and a few examples of its application are provided.
KeywordsDeuterium Protein mass spectrometry Protein dynamics Protein conformation
We would like to thank Dr. Thomas Wales and Dr. Thomas Smithgall for the Lyn SH3:Nef binding data. This work was supported in part by the National Institutes of Health (R01-GM070590 and R01-GM086507). This is contribution 959 from the Barnett Institute.
- 19.Engen JR, Smith DL (2000) Methods Mol Biol 146:95–112Google Scholar