Analytical and Bioanalytical Chemistry

, Volume 396, Issue 4, pp 1599–1606 | Cite as

Influence of salt ions on binding to molecularly imprinted polymers

  • Henrik Kempe
  • Maria KempeEmail author
Original Paper


Salt ions were found to have an influence on template binding to two model molecularly imprinted polymers (MIPs), targeted to penicillin G and propranolol, respectively, in water–acetonitrile mixtures. Water was detrimental to rebinding of penicillin G whereas propranolol bound in the entire water–acetonitrile range tested. In 100% aqueous solution, 3-M salt solutions augmented the binding of both templates. The effects followed the Hofmeister series with kosmotropic ions promoting the largest increase. Binding was mainly of a non-specific nature under these conditions. In acetonitrile containing low amounts of water, the specific binding to the MIPs increased with the addition of salts. Binding of penicillin G followed the Hofmeister series while an ion-exchange mechanism was observed for propranolol. The results suggest that hydration of kosmotropic ions reduces the water activity in water-poor media providing a stabilizing effect on water-sensitive MIP–template interactions. The effects were utilized to develop a procedure for molecularly imprinted solid-phase extraction (MISPE) of penicillin G from milk with a recovery of 87%.


Binding augmentation to a penicillin G imprinted MIP at the addition of kosmotropic ions.


Hofmeister effect Molecular imprinting Molecular recognition Penicillin G Propranolol Solid-phase extraction 



This work was supported by the Swedish Research Council and the Swedish Foundation for Strategic Research (INGVAR, Individual Grant for the Advancement of Research Leaders).

Supplementary material

216_2009_3329_MOESM1_ESM.pdf (847 kb)
ESM 1 (PDF 867 kb)


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Biomedical Polymer Technology, Department of Experimental Medical Science, Biomedical Center, D11Lund UniversityLundSweden

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