Analytical and Bioanalytical Chemistry

, Volume 396, Issue 3, pp 1167–1176 | Cite as

1H NMR-based metabolomics approach for exploring urinary metabolome modifications after acute and chronic physical exercise

  • C. Enea
  • F. Seguin
  • J. Petitpas-Mulliez
  • N. Boildieu
  • N. Boisseau
  • N. Delpech
  • V. Diaz
  • M. Eugène
  • B. DuguéEmail author
Original Paper


Metabolomics is a comprehensive method for metabolite assessment that involves measuring the overall metabolic signature of biological samples. We used this approach to investigate biochemical changes due to acute and chronic physical exercise. Twenty-two women using identical oral contraceptives were segregated into an untrained (n = 10) or trained (n = 12) group depending on their physical training background. The subjects performed two exercises in a randomized order: a prolonged exercise test (75% of their \( \mathop V\limits^\cdot {{\text{O}}_{2\,\;\max }} \) until exhaustion) and a short-term, intensive exercise test (short-term, intensive exercise anaerobic test). Urine specimens were collected before and 30 min after each test. The samples were analyzed by 1H NMR spectroscopy, and multivariate statistical techniques were utilized to process the data. Distinguishing characteristics were observed only in the urine profiles of specimens collected before vs. 30 min after the short-term, intensive exercise test. The metabolites responsible for such changes were creatinine, lactate, pyruvate, alanine, β-hydroxybutyrate, acetate, and hypoxanthine. In both groups, the excretion of lactate, pyruvate, alanine, β-hydroxybutyrate, and hypoxanthine increased similarly after the completion of the short-term, intensive exercise test (p < 0.03). However, acetate excretion increased to a lesser extent in trained than in untrained subjects (p < 0.05). In conclusion, metabolomics is a promising tool in order to gain insight into physiological status and to clarify the changes induced by short-term, intense physical exercise.


Biological variation Metabolomics Physical exercise Pre-analytical factor Urine analysis Young women 



This research was supported by the World Anti-Doping Agency.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • C. Enea
    • 1
  • F. Seguin
    • 2
  • J. Petitpas-Mulliez
    • 1
    • 3
  • N. Boildieu
    • 2
  • N. Boisseau
    • 1
  • N. Delpech
    • 1
  • V. Diaz
    • 1
    • 3
  • M. Eugène
    • 2
    • 3
  • B. Dugué
    • 1
    Email author
  1. 1.Laboratoire des Adaptations Physiologiques aux Activités Physiques (EA 3813), Faculté des Sciences du SportUniversité de PoitiersPoitiersFrance
  2. 2.INSERM, U927, Faculté de Médecine et de PharmacieUniversité de PoitiersPoitiersFrance
  3. 3.Service de PhysiologieCHU PoitiersPoitiersFrance

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