Magnetic-based purification system with simultaneous sample washing and concentration
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Simultaneous washing and concentration of magnetic microparticles was demonstrated using a rotational magnetic system under a continuous-flow condition. The rotation of periodically arranged permanent magnets close to a fluidic channel carrying a suspension of magnetic particles allows the trapping and releasing of particles along the fluidic channel in a periodic manner. Each trapping and releasing event resembles one washing cycle in conventional biological assays. Concentration efficiencies of 99.75 ± 0.083% at a flow rate of 200 µl/min and 88.10 ± 3.17% at a flow rate of 1,000 µl/min and a purification efficiency of 99.10 ± 4.3% at a flow rate of 900 µl/min were achieved.
KeywordsContinuous flow Washing Separation Magnetic particles
This research was supported by A star-Exploit Technologies (ETPL) under Flagship grant no. F07/E/013. The authors thank ETPL and the Institute of Microelectronics for their support.
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- 1.Gijs MAM (2004) Microfluid Nanofluid 1:22–40Google Scholar
- 4.Invitrogen (2008) Dynabeads® CD4. http://tools.invitrogen.com/content/sfs/manuals/111.45_Dynabeads%20CD4.(rev003).pdf
- 16.Zborowski M, Chalmers JJ, Williams PS (2005) In: Cazes J (ed) Encyclopedia of chromatography. CRC, Boca RatonGoogle Scholar