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Analytical and Bioanalytical Chemistry

, Volume 396, Issue 2, pp 825–837 | Cite as

Trace analysis of antidepressants in environmental waters by molecularly imprinted polymer-based solid-phase extraction followed by ultra-performance liquid chromatography coupled to triple quadrupole mass spectrometry

  • Kristof DemeestereEmail author
  • Mira Petrović
  • Meritxell Gros
  • Jo Dewulf
  • Herman Van Langenhove
  • Damià Barceló
Original Paper

Abstract

This paper presents the development, optimization, and validation of an innovative method to analyze trace concentrations of seven selected psychoactive pharmaceuticals in environmental waters. Hereby, the solid-phase extraction (SPE) potential of molecularly imprinted polymers (MIPs) in terms of extraction recovery, breakthrough, precision, and selectivity is studied for the first time. Instrumental analysis by ultra-performance liquid chromatography coupled to triple quadrupole mass spectrometry allowed a rapid (run time = 7.5 min) and sensitive (instrumental detection limit ≤7 pg injected) quantification of the target analytes. A systematic optimization study revealed that, among the seven compounds of interest, mainly the selective serotonin reuptake inhibitors paroxetine, fluoxetine, and citalopram are selectively retained on the MIPs. Experiments performed in spiked river water, sewage treatment plant (STP) effluent and influent showed for these compounds extraction recoveries higher than 70%, breakthrough volumes up to 200 mL, method detection limits (MDL) as low as 0.5 ng/L, and good precision (exemplified by relative standard deviations better than 15%, n ≥ 3). Compared to the widely used hydrophilic–lipophilic balanced (HLB) polymers, the newly developed MIPs indicated to be more resistant toward matrix effects induced ion signal suppression particularly when dealing with relative dirty samples like STP influents. As a result of the better selectivity, the MDL obtained with the MIP-based SPE method was up to a factor of 7 lower compared to those obtained with a recently reported multi-residue HLB method. However, optimizing a HLB method in terms of selectivity, e.g., by introducing a stronger washing protocol, can significantly reduce its MDL up to values approximating those obtained with MIPs.

Keywords

Molecularly imprinted polymers (MIP) Selective serotonin reuptake inhibitors (SSRIs) Benzodiazepines Extraction Environmental water analysis 

Notes

Acknowledgments

The authors acknowledge the financial support for a research stay abroad provided by the Research Foundation —Flanders. Brian Boyd from MIPTechnologies (Lund, Sweden) is greatly thanked for supplying the MISPE cartridges; whereas Waters (Milford, USA) is gratefully acknowledged for providing the Oasis HLB SPE cartridges and the UPLC analytical column.

Supplementary material

216_2009_3270_MOESM1_ESM.pdf (170 kb)
ESM 1 (PDF 170 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kristof Demeestere
    • 1
    Email author
  • Mira Petrović
    • 2
    • 3
  • Meritxell Gros
    • 2
    • 4
  • Jo Dewulf
    • 1
  • Herman Van Langenhove
    • 1
  • Damià Barceló
    • 2
    • 4
  1. 1.Research Group EnVOC, Department of Organic Chemistry, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Department of Environmental ChemistryIDAEA-CSICBarcelonaSpain
  3. 3.Institució Catalana de Recerca i Estudis Avançats (ICREA)BarcelonaSpain
  4. 4.Institut Català de Recerca de l’Aigua (ICRA)Parc Científici Tecnológic de la Universitat de GironaGironaSpain

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