Analytical and Bioanalytical Chemistry

, Volume 396, Issue 2, pp 631–640 | Cite as

Effects of 17β-trenbolone in male eelpout Zoarces viviparus exposed to ethinylestradiol

  • Yohana M. Velasco-Santamaría
  • Steffen S. Madsen
  • Poul Bjerregaard
  • Bodil Korsgaard
Original Paper


To evaluate the interaction between 17β-trenbolone (TB) and 17α-ethinylestradiol (EE2), male eelpout, Zoarces viviparus, was exposed for 21 days (April to May 2008) to 5 ng l−1 EE2 and 5 or 20 ng l−1 TB, separately or in combination in a flow-through SW system. The effects on hepatosomatic (HSI) and gonadosomatic index (GSI), plasma vitellogenin (Vtg) concentration, gonadal histology, hepatic and testicular Vtg mRNA and estrogen receptor (ERα) mRNA expression were investigated. No effects on HSI were observed. A significant decrease was observed in the GSI of all males exposed to EE2 (<0.7%) when compared to controls (1.4%). Histological alterations and immature stages were observed in the testis of all exposed males; however, males exposed to EE2 were the most affected. Increased tubule number and proportionally decreased tubule diameter were observed in the testis of all EE2 groups. No effects in Vtg mRNA expression were observed in the testis; however, a significant decrease in testis ERα mRNA was observed in males exposed to 20 ng l−1 TB. The groups exposed to EE2 showed a significant increase in plasma Vtg (>300-fold), hepatic Vtg mRNA (>450-fold), and ERα mRNA (>100-fold) when compared to controls. This study shows that lower concentrations of 17β-trenbolone are unable to counteract the EE2 estrogenic effects when the exposure is simultaneous.


Microphotograph of testis eelpout (Zoarces viviparus) after 21 days exposure to solvent control (left side) and 5 ng l-1 EE2 (right side). The squares correspond to an area of 500 μm2 and the drawing lines show the longer diameter of the tubules. Sections were 5 μm thickness and stained with H&E.


Eelpout Histology RT–PCR Vitellogenin Trenbolone Ethinylestradiol 



The authors wish to thank Jette Porsgaard and Bente Frost Jacobsen for their skillful technical assistance. The investigation was supported by a grant from the Danish Natural Science Research Council.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yohana M. Velasco-Santamaría
    • 1
  • Steffen S. Madsen
    • 1
  • Poul Bjerregaard
    • 1
  • Bodil Korsgaard
    • 1
  1. 1.Institute of BiologyUniversity of Southern Denmark, OdenseOdense MDenmark

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