Analytical and Bioanalytical Chemistry

, Volume 396, Issue 2, pp 775–781

Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase

Original Paper


A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase.


Peptides Retention mechanisms Surface-confined ionic liquids HPLC Separations/theory 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • K. R. Chitta
    • 1
  • D. S. Van Meter
    • 2
  • A. M. Stalcup
    • 1
  1. 1.Department of ChemistryCincinnatiUSA
  2. 2.Springborn Smithers LaboratoriesWarehamUSA

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