Analytical and Bioanalytical Chemistry

, Volume 396, Issue 1, pp 151–162 | Cite as

Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS

  • Farida Benabdellah
  • Alexandre Seyer
  • Loïc Quinton
  • David Touboul
  • Alain Brunelle
  • Olivier Laprévote
Original Paper


Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.


Mass spectrometry imaging MALDI TOF–SIMS Lipid Rat brain 



This work and the PhD research fellowship of FB are supported by the European Union (contract LSHG-CT2005-518194 COMPUTIS). The PhD research fellowship of AS is supported by the Institut de Chimie des Substances Naturelles (ICSN-CNRS). This work is also supported by the French Agence Nationale pour la Recherche (EICO-CF, grant no. ANR-07-MRAR-006-01).


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Farida Benabdellah
    • 1
  • Alexandre Seyer
    • 1
  • Loïc Quinton
    • 2
  • David Touboul
    • 1
  • Alain Brunelle
    • 1
  • Olivier Laprévote
    • 1
    • 3
  1. 1.Institut de Chimie des Substances NaturellesCNRS UPR 230191198 Gif-sur-Yvette CedexFrance
  2. 2.Laboratoire de spectrométrie de masse, Centre d’Analyse des Résidus en Traces—GIGA-RUniversité de LiègeLiègeBelgium
  3. 3.IFR 71, Faculté de PharmacieUniversité Paris DescartesParisFrance

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