Analytical and Bioanalytical Chemistry

, Volume 396, Issue 1, pp 151–162

Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS

  • Farida Benabdellah
  • Alexandre Seyer
  • Loïc Quinton
  • David Touboul
  • Alain Brunelle
  • Olivier Laprévote
Original Paper

Abstract

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.

Keywords

Mass spectrometry imaging MALDI TOF–SIMS Lipid Rat brain 

References

  1. 1.
    Willoughby D, Cooper DM (2008) Nat Methods 5:29–36CrossRefGoogle Scholar
  2. 2.
    Cruz L, Moroz LL, Gillette R, Sweedler JV (1997) J Neurochem 69:110–115CrossRefGoogle Scholar
  3. 3.
    Freudiger CW, Min W, Saar BG, Lu S, Holtom GR, He C, Tsai JC, Kang JX, Xie XS (2008) Science 322:1857–1861CrossRefGoogle Scholar
  4. 4.
    Greenspan P, Mayer EP, Fowler SD (1985) J Cell Biol 100:965–973CrossRefGoogle Scholar
  5. 5.
    Young LS, Hoffman KR, Stevens P (1975) Proc Soc Exp Biol Med 149:389–396Google Scholar
  6. 6.
    Christie WW (1998) Lipids 33:343–353CrossRefGoogle Scholar
  7. 7.
    Pulfer M, Murphy RC (2003) Mass Spectrom Rev 22:332–364CrossRefGoogle Scholar
  8. 8.
    Karas M, Bachmann D, Bahr U, Hillenkamp F (1987) Int J Mass Spectrom Ion Processes 78:53–68CrossRefGoogle Scholar
  9. 9.
    Hillenkamp F, Peter-Katalinic J (2007) MALDI MS a practical guide to instrumentation, methods and applications. Wiley-VCH, WeinheimGoogle Scholar
  10. 10.
    Castaing R, Slodzian GJ (1962) Microsc 1:395–410Google Scholar
  11. 11.
    Benninghoven A, Loebach E (1971) Rev Sci Instrum 42:49–52CrossRefGoogle Scholar
  12. 12.
    Belu AM, Graham DJ, Castner DG (2003) Biomaterials 24:3635–3653CrossRefGoogle Scholar
  13. 13.
    Li T, Wu TD, Mazéas L, Toffin L, Guerquin-Kern JL, Leblon G, Bouchez T (2008) Environ Microbiol 10:580–588CrossRefGoogle Scholar
  14. 14.
    Touboul D, Halgand F, Brunelle A, Kersting R, Tallarek E, Hagenhoff B, Laprévote O (2004) Anal Chem 76:1550–1559CrossRefGoogle Scholar
  15. 15.
    Sjövall P, Lausmaa J, Johansson B (2004) Anal Chem 76:4271–4278CrossRefGoogle Scholar
  16. 16.
    Touboul D, Kollmer F, Niehuis E, Brunelle A, Laprévote O (2005) J Am Soc Mass Spectrom 16:1608–1618CrossRefGoogle Scholar
  17. 17.
    Weibel D, Wong S, Lockyer N, Blenkinsopp P, Hill R, Vickerman JC (2003) Anal Chem 75:1754–1764CrossRefGoogle Scholar
  18. 18.
    Biddulph GX, Piwowar AM, Fletcher JS, Lockyer NP, Vickerman JC (2007) Anal Chem 79:7259–7266CrossRefGoogle Scholar
  19. 19.
    Cheng J, Kozole J, Hengstebeck R, Winograd N (2007) J Am Soc Mass Spectrom 18:406–412CrossRefGoogle Scholar
  20. 20.
    McDonnell LA, Heeren RMA (2007) Mass Spectrom Rev 26:606–643CrossRefGoogle Scholar
  21. 21.
    Kaletaş BK, van der Wiel IM, Stauber J, Dekker LJ, Güzel C, Kros JM, Luider TM, Heeren RMA (2009) Proteomics 9:2622–2633CrossRefGoogle Scholar
  22. 22.
    Gilmore IS, Seah MP (2002) Appl Surf Sci 187:89–100CrossRefGoogle Scholar
  23. 23.
    Sodhi RN (2004) Analyst 129:483–487CrossRefGoogle Scholar
  24. 24.
    Vickerman JC (2001) TOF–SIMS – an overview. In: Vickerman JC, Briggs D (eds) ToF-SIMS—surface analysis by mass spectrometry. SurfaceSpectra, Manchester, p 1Google Scholar
  25. 25.
    Chaurand P, Schwartz SA, Billheimer D, Xu BJ, Crecelius A, Caprioli RM (2004) Anal Chem 76:1145–1155CrossRefGoogle Scholar
  26. 26.
    Seeley EH, Oppenheimer SR, Mi D, Chaurand P, Caprioli RM (2008) J Am Soc Mass Spectrom 19:1069–1077CrossRefGoogle Scholar
  27. 27.
    Kruse R, Sweedler JV (2003) J Am Soc Mass Spectrom 14:752–759CrossRefGoogle Scholar
  28. 28.
    Sugiura Y, Shimma S, Setou M (2006) Anal Chem 78:8227–8235CrossRefGoogle Scholar
  29. 29.
    Baluya DL, Garrett TJ, Yost RA (2007) Anal Chem 79:6862–6867CrossRefGoogle Scholar
  30. 30.
    Chen Y, Allegood J, Liu Y, Wang E, Cachón-Gonzalez B, Cox TM, Merrill AH Jr, Sullards MC (2008) Anal Chem 80:2780–2788CrossRefGoogle Scholar
  31. 31.
    Aerni HR, Cornett DS, Caprioli RM (2006) Anal Chem 78:827–834CrossRefGoogle Scholar
  32. 32.
    Schuerenberg M, Luebbert C, Deininger SO, Ketterlinus R, Suckau D (2007) Nat Methods 4:iii–ivGoogle Scholar
  33. 33.
    Spengler B, Hubert M (2002) J Am Soc Mass Spectrom 13:735–748CrossRefGoogle Scholar
  34. 34.
    Koestler M, Kirsch D, Hester A, Leisner A, Guenther S, Spengler B (2008) Rapid Commun Mass Spectrom 22:3275–3285CrossRefGoogle Scholar
  35. 35.
    Dreisewerd K, Schurenberg M, Karas M, Hillemkamp F (1995) Int J Mass Spectrom 141:127–148CrossRefGoogle Scholar
  36. 36.
    Touboul D, Brunelle A, Halgand F, De La Porte S, Laprévote O (2005) J Lipid Res 46:1388–1395CrossRefGoogle Scholar
  37. 37.
    Deutsch E (2008) Proteomics 8:2776–2777CrossRefGoogle Scholar
  38. 38.
    Roempp A, Schramm T, Klinkert I, Hester A, Both JP, Brulet M, Brunelle A, Laprévote O, Heeren RMA, Stoeckli M, Spengler B (2009) Proceedings of the 57nd ASMS conference on mass spectrometry and allied topics. Philadelphia, Pennsylvania May 31 – June 4Google Scholar
  39. 39.
    Jana A, Hogan EL, Pahan K (2009) J Neurol Sci 278:5–15CrossRefGoogle Scholar
  40. 40.
    Bougnoux P, Hajjaji N, Couet C (2008) Prostaglandins Leukot Essent Fatty Acids 79:93–96CrossRefGoogle Scholar
  41. 41.
    Holland WL, Summers SA (2008) Endocr Rev 29:381–402CrossRefGoogle Scholar
  42. 42.
    Isaac G, Jeannotte R, Esch SW, Welti R (2007) Genet Eng (N Y) 28:129–157CrossRefGoogle Scholar
  43. 43.
    Touboul D, Piednoël H, Voisin V, De La Porte S, Brunelle A, Halgand F, Laprévote O (2004) Eur J Mass Spectrom 10:657–664CrossRefGoogle Scholar
  44. 44.
    Mazel V, Richardin P, Touboul D, Brunelle A, Walter P, Laprévote O (2006) Anal Chim Acta 570:34–40CrossRefGoogle Scholar
  45. 45.
    Mas S, Touboul D, Brunelle A, Aragoncillo P, Egido J, Laprévote O, Vivanco F (2007) Analyst 132:24–26CrossRefGoogle Scholar
  46. 46.
    Mazel V, Richardin P, Debois D, Touboul D, Cotte M, Brunelle A, Walter P, Laprévote O (2007) Anal Chem 79:9253–9260CrossRefGoogle Scholar
  47. 47.
    Touboul D, Roy S, Germain DP, Chaminade P, Brunelle A, Laprévote O (2007) Int J Mass Spectrom 260:158–165CrossRefGoogle Scholar
  48. 48.
    Mazzucchelli GD, Cellier NA, Mshviladzade V, Elias R, Shim YH, Touboul D, Quinton L, Brunelle A, Laprévote O, De Pauw EA, De Pauw-Gillet MC (2008) J Proteome Res 7:1683–1692CrossRefGoogle Scholar
  49. 49.
    Debois D, Hamze K, Guérineau V, Le Caër JP, Holland IB, Lopes P, Ouazzani J, Séror SJ, Brunelle A, Laprévote O (2008) Proteomics 8:3682–3691CrossRefGoogle Scholar
  50. 50.
    Debois D, Bralet MP, Le Naour F, Brunelle A, Laprévote O (2009) Anal Chem 81:2823–2831CrossRefGoogle Scholar
  51. 51.
    Jackson SN, Wang HY, Woods AS (2007) J Am Soc Mass Spectrom 18:17–26CrossRefGoogle Scholar
  52. 52.
    Eckhardt M (2008) Mol Neurobiol 37:93–103CrossRefGoogle Scholar
  53. 53.
    Vatassery GT, Berry JF, Younozai R, Lam Bergad P (1976) Lipids 11:317–321CrossRefGoogle Scholar
  54. 54.
    Medzihradszky KF, Campbell JM, Baldwin MA, Falick AM, Juhasz P, Vestal ML, Burlingame AL (2000) Anal Chem 72:552–558CrossRefGoogle Scholar
  55. 55.
    Suckau D, Resemann A, Schuerenberg M, Hufnagel P, Franzen J, Holle A (2003) Anal Bioanal Chem 376:952–965CrossRefGoogle Scholar
  56. 56.
    Fletcher JS, Rabbani S, Henderson A, Blenkinsopp P, Thompson SP, Lockyer NP, Vickerman JC (2008) Anal Chem 80:9058–9064CrossRefGoogle Scholar
  57. 57.
    Della-Negra S, Le Beyec Y (1985) Anal Chem 57:2035–2040CrossRefGoogle Scholar
  58. 58.
    Touboul D, Brunelle A, Laprévote O (2006) Rapid Commun Mass Spectrom 20:703–709CrossRefGoogle Scholar
  59. 59.
    Spengler B, Kirsch, Kaufmann R (1991) Rapid Commun Mass Spectrom 5:198–202CrossRefGoogle Scholar
  60. 60.
    Carado A, Passarelli MK, Kozole J, Wingate JE, Winograd N, Loboda AV (2008) Anal Chem 80:7921–7929CrossRefGoogle Scholar
  61. 61.
    Piehowski PD, Carado AJ, Kurczy ME, Ostrowski SG, Heien ML, Winograd N, Ewing AG (2008) Anal Chem 80:8662–8667CrossRefGoogle Scholar
  62. 62.
    Jackson SN, Wang HY, Woods AS, Ugarov M, Egan T, Schultz JA (2005) J Am Soc Mass Spectrom 16:133–138CrossRefGoogle Scholar
  63. 63.
    Jackson SN, Ugarov M, Egan T, Post JD, Langlais D, Albert Schultz J, Woods AS (2007) J Mass Spectrom 42:1093–1098CrossRefGoogle Scholar
  64. 64.
    Fletcher JS, Lockyer NP, Vaidyanathan S, Vickerman JC (2007) Anal Chem 79:2199–2206CrossRefGoogle Scholar
  65. 65.
    Debois D, Brunelle A, Laprévote O (2007) Int J Mass Spectrom 260:115–120CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Farida Benabdellah
    • 1
  • Alexandre Seyer
    • 1
  • Loïc Quinton
    • 2
  • David Touboul
    • 1
  • Alain Brunelle
    • 1
  • Olivier Laprévote
    • 1
    • 3
  1. 1.Institut de Chimie des Substances NaturellesCNRS UPR 230191198 Gif-sur-Yvette CedexFrance
  2. 2.Laboratoire de spectrométrie de masse, Centre d’Analyse des Résidus en Traces—GIGA-RUniversité de LiègeLiègeBelgium
  3. 3.IFR 71, Faculté de PharmacieUniversité Paris DescartesParisFrance

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