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Analytical and Bioanalytical Chemistry

, Volume 395, Issue 2, pp 479–487 | Cite as

Molecularly imprinted monolith in-tube solid-phase microextraction coupled with HPLC/UV detection for determination of 8-hydroxy-2′-deoxyguanosine in urine

  • Shao-Wen Zhang
  • Jun Xing
  • Ling-Shuang Cai
  • Cai-Ying WuEmail author
Original Paper

Abstract

Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) has been widely used as a biomarker of oxidative DNA damage. Measurements of 8-OHdG in urinary samples are challenging owing to the low level of 8-OHdG and the complex matrix. In this study, a novel molecularly imprinted polymer (MIP) monolithic column was synthesized with guanosine as a dummy template which was used as the medium for in-tube solid-phase microextraction (SPME). In-tube SPME coupled with HPLC/UV detection for extraction and determination of urinary 8-OHdG was developed. The synthesized MIP monolithic column exhibited high extraction efficiency owing to its greater phase ratio with convective mass transfer and inherent selectivity. The enrichment factor for 8-OHdG was found to be 76 and the limits of detection and quantification of the method for urinary samples were 3.2 nmol/L (signal-to-noise ratio 3) and 11 nmol/L (signal-to-noise ratio 10), respectively. The MIPs selectivity also made the sample preparation procedure and chromatographic separation much easier. The linear range of the proposed method was from 0.010 to 5.30 μmol/L (r = 0.9997), with a relative standard deviation of 1.1–6.8%, and the recovery for spiked urine samples was 84 ± 3%. The newly developed method was successfully applied to determine urinary samples of healthy volunteers, coking plant workers, and cancer patients. The 8-OHdG level in cancer patients was significantly higher than that in healthy people.

Keywords

Molecularly imprinted monolith In-tube solid-phase microextraction 8-Hydroxy-2′-deoxyguanosine Urine 

Notes

Acknowledgement

We gratefully acknowledge the support of the National Nature Science Foundation of China (grant 20575051).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Shao-Wen Zhang
    • 1
    • 2
  • Jun Xing
    • 1
  • Ling-Shuang Cai
    • 1
    • 3
  • Cai-Ying Wu
    • 1
    Email author
  1. 1.Department of ChemistryWuhan UniversityWuhanChina
  2. 2.Department of Chemistry and Environmental EngineeringLuoyang Institute of Science and TechnologyLuoyangChina
  3. 3.Department of Agricultural and Biosystems EngineeringIowa State UniversityAmesUSA

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