Analytical and Bioanalytical Chemistry

, Volume 395, Issue 3, pp 679–695 | Cite as

Application of microbioreactors in fermentation process development: a review

  • Daniel Schäpper
  • Muhd Nazrul Hisham Zainal Alam
  • Nicolas Szita
  • Anna Eliasson Lantz
  • Krist V. GernaeyEmail author


Biotechnology process development involves strain testing and improvement steps aimed at increasing yields and productivity. This necessitates the high-throughput screening of many potential strain candidates, a task currently mainly performed in shake flasks or microtiter plates. However, these methods have some drawbacks, such as the low data density (usually only end-point measurements) and the lack of control over cultivation conditions in standard shake flasks. Microbioreactors can offer the flexibility and controllability of bench-scale reactors and thus deliver results that are more comparable to large-scale fermentations, but with the additional advantages of small size, availability of online cultivation data and the potential for automation. Current microbioreactor technology is analyzed in this review paper, focusing on its industrial applicability, and directions for future research are presented.


Microbioreactor Fermentation Bioprocess monitoring Microfluidic suspension culture 



The Ph.D. project of Daniel Schäpper is financed by the Novozymes Bioprocess Academy.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Daniel Schäpper
    • 1
  • Muhd Nazrul Hisham Zainal Alam
    • 1
    • 2
  • Nicolas Szita
    • 3
  • Anna Eliasson Lantz
    • 4
  • Krist V. Gernaey
    • 1
    Email author
  1. 1.Department of Chemical and Biochemical EngineeringTechnical University of DenmarkLyngbyDenmark
  2. 2.Department of Bioprocess Engineering, Faculty of Chemical and Natural Resources EngineeringUniversiti Teknologi MalaysiaJohoreMalaysia
  3. 3.Department of Biochemical EngineeringUniversity College LondonLondonUK
  4. 4.Department of Systems BiologyTechnical University of DenmarkLyngbyDenmark

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