Analytical and Bioanalytical Chemistry

, Volume 394, Issue 7, pp 1895–1901

Determination of ethyl-glucuronide in hair for heavy drinking detection using liquid chromatography-tandem mass spectrometry following solid-phase extraction

  • Fabien Lamoureux
  • Jean-michel Gaulier
  • François-Ludovic Sauvage
  • Magali Mercerolle
  • Christine Vallejo
  • Gérard Lachâtre
Original Paper

DOI: 10.1007/s00216-009-2863-0

Cite this article as:
Lamoureux, F., Gaulier, J., Sauvage, FL. et al. Anal Bioanal Chem (2009) 394: 1895. doi:10.1007/s00216-009-2863-0

Abstract

The detection of ethyl-β-d-6-glucuronide (EtG), a stable phase II metabolite of ethanol, is of interest in both clinical and forensic contexts with the aim of monitoring alcohol abuse. We present a liquid chromatography-electrospray ionisation-tandem mass spectrometry method for the detection and quantification of EtG in hair. Thirty milligrams of washed and cut hair were cleaned up using solid-phase extraction graphite cartridges. Separation was then performed using an Uptisphere-3SI column, and the detection was operated in the negative mode. After validation, the method was applied to hair samples taken from four fatalities (F) with documented excessive drinking habits, 12 heavy drinkers (HD) and seven social drinkers (SD). The method exhibits limits of detection and quantification of 4 and 10 pg/mg, respectively. Intra- and inter-assay standard deviation and relative bias were less than 20% over the calibrating range (10 to 3,000 pg/mg). EtG hair concentrations in SD were <10 pg/mg and >50 pg/mg for F and HD (range, 54 to 497 pg/mg). The present assay appears convenient to carry out owing to the very small quantity of hair samples required to determine an effective heavy alcohol consumption (EtG hair concentration >50 pg/mg).

Keywords

Ethyl-glucuronide Hair analysis Liquid chromatography-tandem mass spectrometry Solid-phase extraction Heavy drinking 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Fabien Lamoureux
    • 1
    • 4
  • Jean-michel Gaulier
    • 1
  • François-Ludovic Sauvage
    • 1
  • Magali Mercerolle
    • 1
  • Christine Vallejo
    • 2
  • Gérard Lachâtre
    • 1
    • 3
  1. 1.Department of Pharmacology and ToxicologyUniversity HospitalLimogesFrance
  2. 2.Emergency UnitUniversity HospitalLimogesFrance
  3. 3.Laboratory of ToxicologyFaculty of PharmacyLimogesFrance
  4. 4.Inserm UMR-S850, Laboratoire de Pharmacologie et ToxicologieCentre Hospitalier Universitaire DupuytrenLimoges CedexFrance

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