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Analytical and Bioanalytical Chemistry

, Volume 394, Issue 3, pp 783–789 | Cite as

Surface modification using a novel type I hydrophobin HGFI

  • Sen Hou
  • Xinxin Li
  • Xiaoyu Li
  • Xi-Zeng FengEmail author
  • Rui Wang
  • Chen Wang
  • Lei Yu
  • Ming-Qiang Qiao
Original Paper

Abstract

Surface wettability conversion with hydrophobins is important for its applications in biodevices. In this work, the application of a type I hydrophobin HGFI in surface wettability conversion on mica, glass, and poly(dimethylsiloxane) (PDMS) was investigated. X-ray photoelectron spectroscopy (XPS) and water-contact-angle (WCA) measurements indicated that HGFI modification could efficiently change the surface wettability. Data also showed that self-assembled HGFI had better stability than type II hydrophobin HFBI. Protein patterning and the following immunoassay illustrated that surface modification with HGFI should be a feasible strategy for biosensor device fabrication.

Figure

A hydrophobin HGFI has been applied into surface wettability conversion for protein immobilization

Keywords

Hydrophobin XPS WCA Self-assembly Protein patterning 

Notes

Acknowledgment

This work has been supported by the National Natural Science Foundation of China (Grant number: 90403140).

Supplementary material

216_2009_2776_MOESM1_ESM.pdf (87 kb)
Detailed elemental scans of N1s, C1s, Si2p and Al2p on mica, glass and PDMS surfaces. (DOC 88 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sen Hou
    • 1
  • Xinxin Li
    • 1
  • Xiaoyu Li
    • 1
  • Xi-Zeng Feng
    • 1
    Email author
  • Rui Wang
    • 2
  • Chen Wang
    • 2
  • Lei Yu
    • 3
  • Ming-Qiang Qiao
    • 3
  1. 1.The Key Laboratory of Bioactive Materials, Ministry of EducationCollege of Life Science, Nankai UniversityTianjinChina
  2. 2.National Center for Nanoscience and TechnologyBeijingChina
  3. 3.Institute of Molecular BiologyCollege of Life Science, Nankai UniversityTianjinChina

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