Analytical and Bioanalytical Chemistry

, Volume 394, Issue 3, pp 903–910 | Cite as

Adaptation of the ORAC assay to the common laboratory equipment and subsequent application to antioxidant plastic films

  • K. Bentayeb
  • P. Vera
  • C. Rubio
  • C. NerinEmail author
Original Paper


The oxygen radical absorbance capacity (ORAC) method has been adapted to the instrumental laboratory and optimized for the determination of the antioxidant capacity of a novel active packaging. As the ORAC assay requires the monitorization of a reaction at controlled temperature by means of the fluorescence signal decrease over time, specific instrumental is usually necessary. In this work, a common liquid chromatographic device has been adapted to perform the ORAC assay, leaving it accessible to any laboratory. Using this adaptation, five different essential oils have been determined resulting in the following antioxidant order: clove (2.66 g Trolox per gram of essential oil), oregano (2.25), cinnamon (1.93), rosemary (1.66), and ginger (1.47). After incorporating the essential oils to the film, its antioxidant capacity has also been checked and related to the concentration of essential oil as well as the thickness of the active film. The results point out that for the same amount of essential oil incorporated measured as grams per square meter, thicker films have more antioxidant capacity than the thinner and more concentrated ones. Furthermore, the antioxidant capacity found in the films was always higher than expected taking into account the amount of essential oil incorporated. Some likely explanations have been proposed, leading to the improvement of the antioxidant film under development.


ORAC Antioxidant film Essential oil Active packaging 



This work was financed by the Project INTERREG IIIA- 326-C, the Patronato Cuenca Villoro with the personal grant to K. Bentayeb, and the Project PM18-2007 from Gobierno de Aragón. The authors acknowledge the Company Artibal for supplying the active films.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of Analytical Chemistry i3A, CPSUniversity of ZaragozaZaragossaSpain

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