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Analytical and Bioanalytical Chemistry

, Volume 394, Issue 3, pp 679–686 | Cite as

Bioanalytical applications of SERS (surface-enhanced Raman spectroscopy)

  • Stephen D. Hudson
  • George ChumanovEmail author
Trends

Abstract

Surface-enhanced Raman scattering (SERS) is a powerful technique for analyzing biological samples as it can rapidly and nondestructively provide chemical and, in some cases, structural information about molecules in aqueous environments. In the Raman scattering process, both visible and near-infrared (NIR) wavelengths of light can be used to induce polarization of Raman-active molecules, leading to inelastic light scattering that yields specific molecular vibrational information. The development of surface enhancement has enabled Raman scattering to be an effective tool for qualitative as well as quantitative measurements with high sensitivity and specificity. Recent advances have led to many novel applications of SERS for biological analyses, resulting in new insights for biochemistry and molecular biology, the detection of biological warfare agents, and medical diagnostics for cancer, diabetes, and other diseases. This trend article highlights many of these recent investigations and provides a brief outlook in order to assess possible future directions of SERS as a bioanalytical tool.

Keywords

SERS Optical labels Quantitative SERS Data analysis 

Notes

Acknowledgment

The capped, single Ag NP research from our laboratory was supported by the United States Department of Energy, grant No. DE-FG02-06ER46342.

References

  1. 1.
    Kneipp J, Kneipp H, Kneipp K (2008) SERS—a single-molecule and nanoscale tool for bioanalytics. Chem Soc Rev 37(5):1052–1060PubMedGoogle Scholar
  2. 2.
    Hering K et al. (2008) SERS: a versatile tool in chemical and biochemical diagnostics. Anal Bioanal Chem 390:113–124PubMedGoogle Scholar
  3. 3.
    Barhoumi A, Zhang D, Tam F, Halas NJ (2008) Surface-enhanced Raman spectroscopy of DNA. J Am Chem Soc 130(16):5523–5529PubMedGoogle Scholar
  4. 4.
    Ni F, Sheng RS, Cotton TM (1990) Flow-injection analysis and real-time detection of RNA bases by surface-enhanced Raman-spectroscopy. Anal Chem 62(18):1958–1963PubMedGoogle Scholar
  5. 5.
    Vo-Dinh T (2008) Nanobiosensing using plasmonic nanoprobes. IEEE J Sel Top Quantum Electron 14(1):198–205PubMedPubMedCentralGoogle Scholar
  6. 6.
    Chou IH et al. (2008) Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy. Nano Lett 8(6):1729–1735PubMedPubMedCentralGoogle Scholar
  7. 7.
    Jarvis RM, Goodacre R (2008) Characterisation and identification of bacteria using SERS. Chem Soc Rev 37(5):931–936PubMedGoogle Scholar
  8. 8.
    Primera-Pedrozo OM et al. (2008) Nanotechnology-based detection of explosives and biological agents simulants. IEEE Sens J 8(6):963–973Google Scholar
  9. 9.
    Pearman WF, Fountain AW (2006) Classification of chemical and biological warfare agent simulants by surface-enhanced Raman spectroscopy and multivariate statistical techniques. Appl Spectrosc 60(4):356–365PubMedGoogle Scholar
  10. 10.
    Yan F, Vo-Dinh T (2007) Surface-enhanced Raman scattering detection of chemical and biological agents using a portable Raman integrated tunable sensor. Sens Actuators B 121(1):61–66Google Scholar
  11. 11.
    Banholzer MJ, Millstone JE, Qin LD, Mirkin CA (2008) Rationally designed nanostructures for surface-enhanced Raman spectroscopy. Chem Soc Rev 37(5):885–897PubMedGoogle Scholar
  12. 12.
    Schwartzberg AM, Oshiro TY, Zhang JZ, Huser T, Talley CE (2006) Improving nanoprobes using surface-enhanced Raman scattering from 30-nm hollow gold particles. Anal Chem 78(13):4732–4736PubMedGoogle Scholar
  13. 13.
    Stokes RJ et al. (2008) Surface-enhanced Raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum. Appl Spectrosc 62(4):371–376PubMedGoogle Scholar
  14. 14.
    Ivleva NP, Wagner M, Horn H, Niessner R, Haisch C (2008) In situ surface-enhanced Raman scattering analysis of biofilm. Anal Chem 80(22):8538–8544PubMedGoogle Scholar
  15. 15.
    Lee D et al. (2006) Quantitative analysis of methyl parathion pesticides in a polydimethylsiloxane microfluidic channel using confocal surface-enhanced Raman spectroscopy. Appl Spectrosc 60(4):373–377PubMedGoogle Scholar
  16. 16.
    McLaughlin C, MacMillan D, McCardle C, Smith WE (2002) Quantitative analysis of mitoxantrone by surface-enhanced resonance Raman scattering. Anal Chem 74(13):3160–3167PubMedGoogle Scholar
  17. 17.
    Chen LX, Choo JB (2008) Recent advances in surface-enhanced Raman scattering detection technology for microfluidic chips. Electrophoresis 29(9):1815–1828PubMedGoogle Scholar
  18. 18.
    Podstawka E (2008) Structural properties of bombesin-like peptides revealed by surface-enhanced Raman scattering on roughened silver electrodes. Biopolymers 89(11):980–992PubMedGoogle Scholar
  19. 19.
    Hulteen JC, Vanduyne RP (1995) Nanosphere lithography—a materials general fabrication process for periodic particle array surfaces. J Vac Sci Technol A 13(3):1553–1558Google Scholar
  20. 20.
    Haes AJ, Chang L, Klein WL, Van Duyne RP (2005) Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor. J Am Chem Soc 127(7):2264–2271PubMedGoogle Scholar
  21. 21.
    Haes AJ et al. (2005) Plasmonic materials for surface-enhanced sensing and spectroscopy. MRS Bull 30(5):368–375Google Scholar
  22. 22.
    Stuart DA et al. (2006) In vivo glucose measurement by surface-enhanced Raman spectroscopy. Anal Chem 78(20):7211–7215PubMedGoogle Scholar
  23. 23.
    Zhang XY, Zhao J, Whitney AV, Elam JW, Van Duyne RP (2006) Ultrastable substrates for surface-enhanced Raman spectroscopy: Al2O3 overlayers fabricated by atomic layer deposition yield improved anthrax biomarker detection. J Am Chem Soc 128(31):10304–10309PubMedGoogle Scholar
  24. 24.
    Abdelsalam M (2008) Quantitative electrochemical SERS of flavin at a structured silver surface. Langmuir 24(13):7018–7023PubMedGoogle Scholar
  25. 25.
    Driskell JD et al. (2008) Infectious agent detection with SERS-active silver nanorod arrays prepared by oblique angle deposition. IEEE Sens J 8(6):863–870Google Scholar
  26. 26.
    Driskell JD et al. (2008) Rapid microRNA (miRNA) detection and classification via surface-enhanced Raman spectroscopy (SERS). Biosens Bioelectron 24(4):923–928PubMedGoogle Scholar
  27. 27.
    Fang C et al. (2008) DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label. Biosens Bioelectron 24(2):216–221PubMedGoogle Scholar
  28. 28.
    Lin M et al. (2008) Detection of melamine in gluten, chicken feed, and processed foods using surface enhanced Raman spectroscopy and HPLC. J Food Sci 73(8):T129–T134PubMedGoogle Scholar
  29. 29.
    Daniels JK, Caldwell TP, Christensen KA, Chumanov G (2006) Monitoring the kinetics of Bacillus subtilis endospore germination via surface-enhanced Raman scattering spectroscopy. Anal Chem 78(5):1724–1729Google Scholar
  30. 30.
    Evanoff DD, Heckel J, Caldwell TP, Christensen KA, Chumanov G (2006) Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy. J Am Chem Soc 128(39):12618–12619PubMedGoogle Scholar
  31. 31.
    Smith WE (2008) Practical understanding and use of surface enhanced Raman scattering/surface enhanced resonance Raman scattering in chemical and biological analysis. Chem Soc Rev 37(5):955–964PubMedGoogle Scholar
  32. 32.
    Etchegoin PG, Le Ru EC (2008) A perspective on single molecule SERS: current status and future challenges. Phys Chem Chem Phys 10(40):6079–6089PubMedGoogle Scholar
  33. 33.
    Bell SEJ, Mackle JN, Sirimuthu NMS (2005) Quantitative surface-enhanced Raman spectroscopy of dipicolinic acid—towards rapid anthrax endospore detection. Analyst 130(4):545–549PubMedGoogle Scholar
  34. 34.
    Yeo BS, Schmid T, Zhang WH, Zenobi R (2008) A strategy to prevent signal losses, analyte decomposition, and fluctuating carbon contamination bands in surface-enhanced Raman spectroscopy. Appl Spectrosc 62(6):708–713PubMedGoogle Scholar
  35. 35.
    Levin CS et al. (2008) Interactions of ibuprofen with hybrid lipid bilayers probed by complementary surface-enhanced vibrational spectroscopies. J Phys Chem B 112(45):14168–14175PubMedPubMedCentralGoogle Scholar
  36. 36.
    Ozaki Y et al. (2008) Simplified protocol for detection of protein-ligand interactions via surface-enhanced resonance Raman scattering and surface-enhanced fluorescence. Anal Chem 80(17):6567–6572PubMedGoogle Scholar
  37. 37.
    Porter MD, Lipert RJ, Siperko LM, Wang G, Narayanana R (2008) SERS as a bioassay platform: fundamentals, design, and applications. Chem Soc Rev 37(5):1001–1011PubMedGoogle Scholar
  38. 38.
    Doering WE, Piotti ME, Natan MJ, Freeman RG (2007) SERS as a foundation for nanoscale, optically detected biological labels. Adv Mater 19(20):3100–3108Google Scholar
  39. 39.
    Sun L et al. (2007) Composite organic-inorganic nanoparticles as Raman labels for tissue analysis. Nano Lett 7(2):351–356PubMedGoogle Scholar
  40. 40.
    Huang XH, El-Sayed IH, Qian W, El-Sayed MA (2007) Cancer cells assemble and align gold nanorods conjugated to antibodies to produce highly enhanced, sharp, and polarized surface Raman spectra: a potential cancer diagnostic marker. Nano Lett 7(6):1591–1597PubMedGoogle Scholar
  41. 41.
    Keren S et al. (2008) Noninvasive molecular imaging of small living subjects using Raman spectroscopy. Proc Natl Acad Sci USA 105(15):5844–5849Google Scholar
  42. 42.
    Kim K, Lee YM, Lee HS, Shin KS (2008) The utilization of silver salts of aromatic thiols as core materials of SERS-based molecular sensors. J Raman Spectrosc 39:1840–1847Google Scholar
  43. 43.
    Hudson S, Chumanov G (2008) SERS and resonance elastic scattering from capped single Ag nanoparticles. J Phys Chem C (accepted)Google Scholar
  44. 44.
    Kneipp K, Kneipp H, Kneipp J (2006) Surface-enhanced Raman scattering in local optical fields of silver and gold nanoaggregates: from single-molecule Raman spectroscopy to ultrasensitive probing in live cells. Acc Chem Res 39(7):443–450PubMedGoogle Scholar
  45. 45.
    Tang HW, Yang XBB, Kirkham J, Smith DA (2008) Chemical probing of single cancer cells with gold nanoaggregates by surface-enhanced Raman scattering. Appl Spectrosc 62(10):1060–1069PubMedGoogle Scholar
  46. 46.
    Tang HW, Yang XB, Kirkham J, Smith DA (2007) Probing intrinsic and extrinsic components in single osteosarcoma cells by near-infrared surface-enhanced Raman scattering. Anal Chem 79(10):3646–3653PubMedGoogle Scholar
  47. 47.
    Adar F, FitzGerald S, Morel S, Whitley A (2006) Raman microscopy extends frontiers of biomedical research. Biophotonics Int 13(3):44–49Google Scholar
  48. 48.
    Liu XJ, Huan SY, Bu YL, Shen GL, Yu RQ (2008) Liposome-mediated enhancement of the sensitivity in immunoassay based on surface-enhanced Raman scattering at gold nanosphere array substrate. Talanta 75(3):797–803PubMedGoogle Scholar
  49. 49.
    Li T, Guo LP, Wang ZX (2008) Microarray based Raman spectroscopic detection with gold nanoparticle probes. Biosens Bioelectron 23(7):1125–1130PubMedGoogle Scholar
  50. 50.
    Sabatte G et al. (2008) Comparison of surface-enhanced resonance Raman scattering and fluorescence for detection of a labeled antibody. Anal Chem 80(7):2351–2356PubMedGoogle Scholar
  51. 51.
    Han XX et al. (2008) Fluorescein isothiocyanate linked immunoabsorbent assay based on surface-enhanced resonance Raman scattering. Anal Chem 80(8):3020–3024PubMedGoogle Scholar
  52. 52.
    Monaghan PB et al. (2007) Bead-based DNA diagnostic assay for chlamydia using nanoparticle-mediated surface-enhanced resonance Raman scattering detection within a lab-on-a-chip format. Anal Chem 79(7):2844–2849PubMedGoogle Scholar
  53. 53.
    Lim IIS et al. (2008) Gold and magnetic oxide/gold core/shell nanoparticles as bio-functional nanoprobes. Nanotechnology 19:305102PubMedGoogle Scholar
  54. 54.
    Qian XM, Zhou X, Nie SM (2008) Surface-enhanced Raman nanoparticle beacons based on bio-conjugated gold nanocrystals and long range plasmonic coupling. J Am Chem Soc 130(45):14934PubMedPubMedCentralGoogle Scholar
  55. 55.
    Budich C, Neugebauer U, Popp J, Deckert V (2008) Cell wall investigations utilizing tip-enhanced Raman scattering. J Microsc–Oxford 229(3):533–539Google Scholar
  56. 56.
    Yeo BS, Madler S, Schmid T, Zhang WH, Zenobi R (2008) Tip-enhanced Raman spectroscopy can see more: the case of cytochrome C. J Phys Chem C 112(13):4867–4873Google Scholar
  57. 57.
    Rasmussen A, Deckert V (2006) Surface- and tip-enhanced Raman scattering of DNA components. J Raman Spectrosc 37(1–3):311–317Google Scholar
  58. 58.
    Bailo E, Deckert V (2008) Tip-enhanced Raman spectroscopy of single RNA strands: towards a novel direct-sequencing method. Angew Chem Int Ed 47(9):1658–1661Google Scholar
  59. 59.
    Domke KF, Zhang D, Pettinger B (2007) Tip-enhanced Raman spectra of picomole quantities of DNA nucleobases at Au(111). J Am Chem Soc 129(21):6708PubMedGoogle Scholar
  60. 60.
    Steidtner J, Pettinger B (2008) Tip-enhanced Raman spectroscopy and microscopy on single dye molecules with 15 nm resolution. Phys Rev Lett 100(23):236101PubMedGoogle Scholar
  61. 61.
    Schmid T, Messmer A, Yeo BS, Zhang WH, Zenobi R (2008) Towards chemical analysis of nanostructures in biofilms II: tip-enhanced Raman spectroscopy of alginates. Anal BioAnal Chem 391(5):1907–1916PubMedGoogle Scholar
  62. 62.
    Bailo E, Deckert V (2008) Tip-enhanced Raman scattering. Chem Soc Rev 37(5):921–930PubMedGoogle Scholar
  63. 63.
    Jarvis RM, Brooker A, Goodacre R (2006) Surface-enhanced Raman scattering for the rapid discrimination of bacteria. Faraday Disc 132:281–292Google Scholar
  64. 64.
    Lutz B, Knudsen B (2008) Spectral analysis of multiplex Raman probe signatures. ACS Nano 2(11):2306–2314PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.235 H.L. Hunter LaboratoriesClemsonUSA

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