Analytical and Bioanalytical Chemistry

, Volume 394, Issue 2, pp 523–528 | Cite as

Pollen-imprinted polyurethanes for QCM allergen sensors

  • Michael Jenik
  • Alexandra Seifner
  • Peter Lieberzeit
  • Franz L. DickertEmail author
Original Paper


Molecularly imprinted polymers for detecting plant pollen were designed as artificial recognition materials for quartz crystal microbalances in the gaseous phase. Imprints of birch (diameter, 25 μm) and nettle (diameter, 15 μm) pollen can be generated by polydimethylsiloxane stamping technique as proven by atomic force microscopy. If pollen grains are able to access the cavities and thus are incorporated, the resulting sensors display Sauerbrey-like negative frequency shifts. Non-Sauerbrey behaviour can be observed as soon as pollen is prevented from entering the selective hollows: this results in grain mobility on the electrode surface leading to frequency increases. Access to the cavities is determined by the diameter ratio between pollen grains and imprints as can be revealed during cross-selectivity measurements of nettle and birch pollen imprinted layers. When the amount of pollen grains on the electrode surface exceeds the number of available imprints, the excess particles move freely, resulting in positive, non-Sauerbrey frequency shifts.


Molecular imprinting Plant pollen QCM Non-Sauerbrey behaviour 


  1. 1.
    Arnau A (2008) Sensors 8:370–411CrossRefGoogle Scholar
  2. 2.
    O’Sullivan CK, Guilbault GG (1999) Biosens and Bioelectron 14:663–670CrossRefGoogle Scholar
  3. 3.
    Pejcic B, Eadington P, Ross A (2007) Environ Sci and Technol 41:6333–6342CrossRefGoogle Scholar
  4. 4.
    Lieberzeit PA, Dickert FL (2007) Anal and Bioanal Chemistry 387:237–247CrossRefGoogle Scholar
  5. 5.
    Sauerbrey G (1959) Z Phys 155:206–222CrossRefGoogle Scholar
  6. 6.
    Marx KA (2003) Biomacromolecules 4:1099–1120CrossRefGoogle Scholar
  7. 7.
    Davis F, Higson SPJ (2005) Biosens Bioelectron 21:1–20CrossRefGoogle Scholar
  8. 8.
    Lieberzeit PA, Afzal A, Podlipna D, Krassnig S, Blumenstock H, Dickert FL (2007) Sens Actuators B 126:153–158CrossRefGoogle Scholar
  9. 9.
    Ye L, Haupt K (2004) Anal Bioanal Chem 378:1887–1897CrossRefGoogle Scholar
  10. 10.
    Belmont AS, Jaeger S, Knopp D, Niessner R, Gauglitz G, Haupt K (2007) Biosens Bioelectron 12:3267–3272CrossRefGoogle Scholar
  11. 11.
    Alexander C, Vulfson EN (1997) Adv Mater 9:751–755CrossRefGoogle Scholar
  12. 12.
    Wink TH, Van Zuilen SJ, Bult A, Van Bennekom WP (1997) Analyst 122:43R–50RCrossRefGoogle Scholar
  13. 13.
    Lieberzeit PA, Glanznig G, Jenik M, Gazda-Miarecka S, Dickert FL, Leidl A (2005) Sensors 5:509–518CrossRefGoogle Scholar
  14. 14.
    Wiermann R, Gubatz S (1992) Int Rev Cytol 140:35–72CrossRefGoogle Scholar
  15. 15.
    Scott RJ (1994) Molecular and cellular aspects of plant reproduction. Cambridge University PressGoogle Scholar
  16. 16.
    Ahlers F, Thom I, Lambert J, Kuckuk R, Wiermann R (1999) Phytochem 50:1095–1098CrossRefGoogle Scholar
  17. 17.
    Jungfernmann C, Ahlers F, Grote M, Gubatz S, Steuernagel S et al (1997) J Plant Physiol 151:513–519Google Scholar
  18. 18.
    Espelie KE, Loewus FA, Pugmire RJ, Woolfenden WR, Baldi BG, Given PH (1989) Phytochem 28:751–753CrossRefGoogle Scholar
  19. 19.
    Guilford WJ, Schneider DM, Labowitz J, Opella SJ (1988) Plant Physiol 86:134–136CrossRefGoogle Scholar
  20. 20.
    Wilmesmeier S, Steuernagel S, Wiermann R (1993) Z Naturforsch 48c:697–701Google Scholar
  21. 21.
    Fritsch R, Bohle B, Vollmann U, Wiedermann U, Jahn-Schmid B, Krebitz M, Breiteneder H, Kraft D, Ebner C (1998) J Allergy and Clin Immunol 102:679–686CrossRefGoogle Scholar
  22. 22.
    Dickert FL, Hayden O (2002) Anal Chem 74:1302–130CrossRefGoogle Scholar
  23. 23.
    Hayden O, Mann KJ, Krassnig S, Dickert FL (2006) Angew Chem Int Ed 45:2626–2629CrossRefGoogle Scholar
  24. 24.
    Lieberzeit PA, Schirk C, Glanznig G, Gazda-Miarecka S, Bindeus R, Nannen H, Kauling J, Dickert FL (2004) Superlattices Microstruct 36:133–142CrossRefGoogle Scholar
  25. 25.
    McHale G, Lucklum R, Newton MI, Cowen JA (2000) J Appl Phys 88:7304–7312CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Michael Jenik
    • 1
  • Alexandra Seifner
    • 1
  • Peter Lieberzeit
    • 1
  • Franz L. Dickert
    • 1
    Email author
  1. 1.Department of Analytical Chemistry and Food ChemistryUniversity of ViennaViennaAustria

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