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Analytical and Bioanalytical Chemistry

, Volume 393, Issue 8, pp 2009–2018 | Cite as

Determination of trace tetracycline antibiotics in foodstuffs by liquid chromatography–tandem mass spectrometry coupled with selective molecular-imprinted solid-phase extraction

  • Tao Jing
  • Xiao-Dan Gao
  • Peng Wang
  • Yan Wang
  • Yan-Fei Lin
  • Xiao-Zhong Hu
  • Qiao-Lin Hao
  • Yi-Kai Zhou
  • Su-Rong MeiEmail author
Original Paper

Abstract

A rapid, specific, and sensitive method has been developed using molecularly imprinted polymers (MIPs) as solid-phase extraction sorbents for extraction of trace tetracycline antibiotics (TCs) in foodstuffs. MIPs were prepared by precipitation polymerization using tetracycline as the template. Under the optimal condition, the imprinting factors for MIPs were 4.1 (oxytetracycline), 7.0 (tetracycline), 7.4 (chlortetracycline), 7.7 (doxycycline), respectively. Furthermore, the performance of MIPs as solid-phase extraction sorbents was evaluated and high extraction efficiency of molecularly imprinted solid-phase extraction (MISPE) procedure was demonstrated. Compared with commercial sorbents, MISPE gave a better cleanup efficiency than C18 cartridge and a higher recovery than Oasis HLB cartridge. Finally, the method of liquid chromatography–tandem mass spectrometry coupled with molecular-imprinted solid-phase extraction was validated in real samples including lobster, duck, honey, and egg. The spiked recoveries of TCs ranged from 94.51% to 103.0%. The limits of detection were in the range of 0.1–0.3 μg kg−1.

Chromatograms obtained by direct injection of the spiked egg extracts (5 × 10-3 mmol L−1) and purification with MISPE

Keywords

Tetracycline antibiotics Precipitation polymerization Molecularly imprinted solid-phase extraction Foodstuff Liquid chromatography–tandem mass spectrometry 

Notes

Acknowledgement

This work was supported by the National Nature Science Foundation of China (grant no. 20407009, 30771807) and the Entry–Exit Inspection and Quarantine Bureau of PRC (grant no. 2006IK152).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Tao Jing
    • 1
  • Xiao-Dan Gao
    • 1
  • Peng Wang
    • 2
  • Yan Wang
    • 1
  • Yan-Fei Lin
    • 2
  • Xiao-Zhong Hu
    • 2
  • Qiao-Lin Hao
    • 1
  • Yi-Kai Zhou
    • 1
  • Su-Rong Mei
    • 1
    Email author
  1. 1.MOE Key Laboratory of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Hubei Entry–Exit Inspection and Quarantine Bureau of PRCWuhanChina

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