Two-step liquid-phase microextraction and high-performance liquid chromatography for the simultaneous analysis of the enantiomers of mefloquine and its main metabolite carboxymefloquine in plasma

  • Igor Rafael dos Santos Magalhães
  • Pierina Sueli Bonato
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

A method for the simultaneous analysis of the enantiomers of mefloquine (MQ) and its main metabolite carboxymefloquine (CMQ) in plasma is described for the first time. The assay involves two-step liquid-phase microextraction (LPME) and enantioselective high-performance liquid chromatography. In the first LPME step, the enantiomers of MQ were extracted from an alkalinized sample through a thin layer of di-n-hexyl ether immobilized in the pores of the hollow fiber and into 0.01 M perchloric acid as acceptor solution. In the second LPME step, the same sample was acidified to enable the extraction of CMQ using the same organic solvent and 0.05 M sodium hydroxide as acceptor phase. The analytes were resolved on a Chirobiotic T column in the polar-organic mode of elution and detected at 285 nm. The recovery rates from 1 mL of plasma were in the range 35–38%. The method presented limits of quantification of 50 ng/mL for all analytes and was linear up to 1,500 and 3,000 ng/mL for the enantiomers of MQ and CMQ, respectively. The plasmatic concentrations of (+)-(RS)-MQ were higher than those of (−)-(SR)-MQ after oral administration of the racemic drug to rats.

Extraction device for LPME employing hollow fiber membranes used in the developed method

Keywords

Mefloquine enantiomers Carboxymefloquine Liquid-phase microextraction Liquid chromatography Plasma samples 
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Notes

Acknowledgments

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for granting financial support and research fellowships.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Igor Rafael dos Santos Magalhães
    • 1
  • Pierina Sueli Bonato
    • 1
  1. 1.Faculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil

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