Fluorescence properties of selected benzo[c]phenantridine alkaloids and studies of their interaction with CT DNA

  • Jana Urbanová
  • Přemysl Lubal
  • Iva Slaninová
  • Eva Táborská
  • Petr Táborský
Original Paper
First Online:
Received:
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Abstract

The spectral, especially fluorescence properties, of seven selected quaternary benzo[c]phenantridine alkaloids (sanguinarine, chelerythrine, chelirubine, sanguirubine, chelilutine, sanguilutine, and macarpine) were studied in presence and in absence of double-stranded DNA. This study has proved dramatic differences in fluorescence emission of all studied alkaloids in presence of calf thymus DNA in comparison to fluorescence of free alkaloids. The most remarkable are changes in emission spectra of macarpine, chelirubine, and sanguirubine. Association constants (logK) for interaction of all studied alkaloids with CT DNA were calculated.

Keywords

Benzo[c]phenantridine alkaloids Sanguinarine Chelirubine Sanguirubine Chelilutine Macarpine Association constants 
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Notes

Acknowledgments

This work was supported by projects CSF 525/08/0819 and LC06035 BIO-ANAL-MED.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jana Urbanová
    • 1
  • Přemysl Lubal
    • 1
  • Iva Slaninová
    • 2
  • Eva Táborská
    • 3
  • Petr Táborský
    • 1
  1. 1.Department of Chemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Department of Biology, Faculty of MedicineMasaryk UniversityBrnoCzech Republic
  3. 3.Department of Chemistry and Biochemistry, Faculty of MedicineMasaryk UniversityBrnoCzech Republic

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