Analytical and Bioanalytical Chemistry

, Volume 393, Issue 6–7, pp 1763–1768 | Cite as

Selective solid-phase extraction of ethynylestradiol from river water by molecularly imprinted polymer microcolumns

  • J. C. Bravo
  • R. M. GarcinuñoEmail author
  • P. Fernández
  • J. S. Durand
Original Paper


A selective on-column molecularly imprinted solid-phase extraction (MISPE) for ethynylestradiol (EE2) from water samples was developed. Following a non-covalent molecular imprinting approach, two molecularly imprinted polymers (MIPs) have been synthesised using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker and EE2 as template, in two different polymerisation solvents (acetonitrile or toluene). The optimisation of the on-column MISPE conditions for the selective rebinding of the EE2 resulted in a flow rate of 2.3 mL min−1 and volumes of 1 mL toluene and 1.5 mL methanol–acetic acid (9:1) for washing and elution steps, respectively. The selectivity of the imprinted polymer towards several related estrogens such as estriol (E3), estrone (E1) and estradiol (E2) was evaluated. The recovery of EE2 from a 50-mL spiked river-water sample was 75% when the optimised on-column MISPE was applied.


Ethynylestradiol Estrogens MIPs SPE Waters 



This work has been supported by project S-0505/AGR/0312 (Spanish Government, Comunidad de Madrid).


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • J. C. Bravo
    • 1
  • R. M. Garcinuño
    • 1
    Email author
  • P. Fernández
    • 1
  • J. S. Durand
    • 1
  1. 1.Departamento de Ciencias Analíticas, Facultad de CienciasUniversidad Nacional de Educación a DistanciaMadridSpain

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