Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women

  • M.-L. Bayle
  • C. Enea
  • P. Goetinck
  • F. Lafay
  • N. Boisseau
  • B. Dugué
  • M.-M. Flament-WatonEmail author
  • M.-F. Grenier-Loustalot
Original Paper


Dehydroepiandrosterone (DHEA) and its metabolite androsterone (A) are natural steroids secreted in high quantities in human body. To assess the influence of oral contraceptives, menstrual cycle phase, and also physical exercise (acute and chronic such as training) on these metabolites excretions, a collection of 28 female urine specimens was organized. A three-extraction-step method was developed, and the analyses were performed by gas chromatography–mass spectrometry using deuterated 19-noretiocholanolone as the internal standard. Sample hydration state was found to be of great importance for kinetic studies, as it directly influenced the concentrations. No influence of menstrual cycle and training was found for androsterone and DHEA. However, oral contraceptive intake lowered DHEA excretion in urine and A seems to be slightly affected by exercise.


Androsterone Dehydroepiandrosterone Doping Female physiology Gas chromatography–mass spectrometry Physical exercise 



The authors thank the World Anti-Doping Agency (WADA) for the financial support.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • M.-L. Bayle
    • 1
  • C. Enea
    • 2
  • P. Goetinck
    • 1
  • F. Lafay
    • 1
  • N. Boisseau
    • 2
  • B. Dugué
    • 2
  • M.-M. Flament-Waton
    • 1
    Email author
  • M.-F. Grenier-Loustalot
    • 1
  1. 1.Service Central d’Analyse (USR 59—CNRS)Echangeur de SolaizeSolaizeFrance
  2. 2.Laboratoire des Adaptations Physiologiques aux Activités Physiques (EA3813)Université de PoitiersPoitiersFrance

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