Analytical and Bioanalytical Chemistry

, Volume 392, Issue 7–8, pp 1277–1282

Spatial distribution of heme species in erythrocytes infected with Plasmodium falciparum by use of resonance Raman imaging and multivariate analysis

  • Alois Bonifacio
  • Sara Finaurini
  • Christoph Krafft
  • Silvia Parapini
  • Donatella Taramelli
  • Valter Sergo
Technical Note


The multivariate algorithm hierarchical cluster analysis is applied to sets of resonance Raman spectra collected from human erythrocytes infected with the malaria parasite Plasmodium falciparum. The images obtained yield information about the distribution of hemoglobin and hemozoin (or malaria pigment) within the parasitized cells and about their molecular structure. This method has the advantage of conveying more information than other imaging approaches based on resonance Raman spectroscopy, and it is a promising tool to study the hemozoin formation process and its interaction with antimalarial drugs within unstained, well-preserved parasites.


Malaria Plasmodium falciparum Hemozoin Imaging Resonance Raman Multivariate analysis 

Supplementary material

216_2008_2414_MOESM1_ESM.pdf (101 kb)
ESM(PDF 100 kb)


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Alois Bonifacio
    • 1
  • Sara Finaurini
    • 2
  • Christoph Krafft
    • 3
  • Silvia Parapini
    • 2
  • Donatella Taramelli
    • 2
  • Valter Sergo
    • 1
  1. 1.Department of Materials and Natural ResourcesUniversity of TriesteTriesteItaly
  2. 2.Department of Public Health-Microbiology-VirologyUniversity of MilanMilanItaly
  3. 3.Bioanalytical ChemistryDresden University of TechnologyDresdenGermany

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