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New technologies in affinity assays to explore biological communication

  • Michael G. Roper
  • Christelle Guillo
Trends

Abstract

The language that cells use to communicate consists of the small molecules, peptides, and proteins that are released into the extracellular environment. To decipher this language, analytical assays are needed that have high selectivity, high sensitivity, and fast temporal resolution. Affinity assays are a group of analytical methodologies that are adept at studying this communication. In this overview, we highlight several examples from the literature on various types of affinity assays used in different platforms to monitor biological communication of peptides and proteins.

Keywords

Bioanalytical methods Immunoassays/ELISA PCR Microfluidics/microfabrication Capillary electrophoresis Affinity 

Notes

Acknowledgements

MGR acknowledges support from NIH R01 DK080714 and the American Heart Association Greater Southeast Affiliate.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryFlorida State UniversityTallahasseeUSA

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