Capillary electrophoresis–mass spectrometry in urinary proteome analysis: current applications and future developments

  • Jochen MetzgerEmail author
  • Joost P. Schanstra
  • Harald Mischak


The development of noninvasive methods for diagnosis and prognosis of many diseases still remains a challenging task in clinical medicine. In recent years, protein separation and detection techniques have been refined such that they allow proteome screening of biological fluids with good reproducibility, accuracy, and real-time performance. The advancements in this field resulted in the initiation and conduction of many clinical studies aimed towards defining sensitive and selective biomarkers for disease or a pathological condition. In this review, we focus on the description of the state of the art proteomic platforms established or adapted to use urine as sample source for biomarker discovery. In the second part of this review, we will give an overview of recent clinical studies that used capillary electrophoresis–mass spectrometry (CE-MS) in urinary proteomics/peptidomics. Owing to its fast and accurate one-step screening method CE-MS allows resolution of the urinary small molecular weight proteome in high-throughput mode. This makes CE-MS well suited for use in large proteomic studies. The fact that urinary proteomic studies are not restricted to renal and urological disorders, but also enable assessment of systemic diseases like cardiovascular or infectious diseases, clearly demonstrates the considerably high potential that urine offers for biomarker discovery and for diagnosis, staging, prognosis, and progression monitoring of diseases.


Biomarker discovery Capillary electrophoresis–mass spectrometry Urinary proteome analysis 



two-dimensional electrophoresis


benign prostatic hyperplasia


coronary artery disease


capillary electrophoresis


CE-coupled mass spectrometry


chronic kidney disease


differential in-gel electrophoresis


diabetic nephropathy


electroosmotic flow


electrospray time-of-flight mass spectrometer


end-stage renal disease


focal segmental glomerulosclerosis


graft versus host disease


hematopoietic stem cell transplantation


IgA nephropathy


liquid chromatography


minimal change disease


membranous glomerulonephritis


mass spectrometry


multidimensional protein identification technology


prostate cancer


sodium dodecylsulfate


surface-enhanced laser desorption/ionization


ureteropelvic junction obstruction


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jochen Metzger
    • 1
    Email author
  • Joost P. Schanstra
    • 2
    • 3
  • Harald Mischak
    • 1
  1. 1.Mosaiques diagnostics & therapeuticsHannoverGermany
  2. 2.Department of Renal and Cardiac RemodellingInserm, U858/I2MR, Team #5Toulouse Cedex 4France
  3. 3.Institut de Médecine Moléculaire de RangueilUniversité Toulouse III Paul SabatierToulouseFrance

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