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Analytical and Bioanalytical Chemistry

, Volume 392, Issue 6, pp 1059–1073 | Cite as

Engineering and assaying of cytochrome P450 biocatalysts

  • Kersten S. Rabe
  • Valérie J. Gandubert
  • Mark Spengler
  • Michael Erkelenz
  • Christof M. Niemeyer
Review

Abstract

Cytochrome P450s constitute a highly fascinating superfamily of enzymes which catalyze a broad range of reactions. They are essential for drug metabolism and promise industrial applications in biotechnology and biosensing. The constant search for cytochrome P450 enzymes with enhanced catalytic performances has generated a large body of research. This review will concentrate on two key aspects related to the identification and improvement of cytochrome P450 biocatalysts, namely the engineering and assaying of these enzymes. To this end, recent advances in cytochrome P450 development are reported and commonly used screening methods are surveyed.

Keywords

Analytical methods Cytochrome P450 Enzyme engineering High-throughput screening P450 substrates 

Notes

Acknowledgments

This work was supported by the Zentrum für Angewandte Chemische Genomik, a joint research initiative founded by the European Union and the Ministry of Innovation and Research of the state of North Rhine Westfalia.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Kersten S. Rabe
    • 1
  • Valérie J. Gandubert
    • 1
  • Mark Spengler
    • 1
  • Michael Erkelenz
    • 1
  • Christof M. Niemeyer
    • 1
    • 2
  1. 1.Fakultät für Chemie, Biologisch-Chemische MikrostrukturtechnikTechnische Universität DortmundDortmundGermany
  2. 2.ISAS-Institute for Analytical SciencesDortmundGermany

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