Analytical and Bioanalytical Chemistry

, Volume 391, Issue 8, pp 2905–2914 | Cite as

Development of a selective molecularly imprinted polymer-based solid-phase extraction for indomethacin from water samples

  • Tao Yang
  • Ya-Hui Li
  • Shuang Wei
  • Yuan Li
  • Anping DengEmail author
Original Paper


A selective molecularly imprinted solid-phase extraction (MISPE) for indomethacin (IDM) from water samples was developed. Using IDM as template molecule, acrylamide (AM) or methacrylic acid (MAA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, and bulk or suspension polymerization as the synthetic method, three molecularly imprinted polymers (MIPs) were synthesized and characterized with a rebinding experiment. It was found that the MIP of AM-EDMA produced by bulk polymerization showed the highest binding capacity for IDM, and so it was chosen for subsequent experiments, such as those testing the selectivity and recognition binding sites. Scatchard analysis revealed that at least two kinds of binding sites formed in the MIP, with the dissociation constants of 7.8 μmol L−1 and 127.2 μmol L−1, respectively. Besides IDM, three structurally related compounds — acemetacin, oxaprozin and ibuprofen — were employed for selectivity tests. It was observed that the MIP exhibited the highest selective rebinding to IDM. Accordingly, the MIP was used as a solid-phase extraction sorbent for the extraction and enrichment of IDM in water samples. The extraction conditions of the MISPE column for IDM were optimized to be: chloroform or water as loading solvent, chloroform with 20% acetonitrile as washing solution, and methanol as eluting solvent. Water samples with or without spiking were extracted by the MISPE column and analyzed by HPLC. No detectable IDM was observed in tap water and the content of IDM in a river water sample was found to be 1.8 ng mL−1. The extraction efficiencies of the MISPE column for IDM in spiked tap and river water were acceptable (87.2% and 83.5%, respectively), demonstrating the feasibility of the prepared MIP for IDM extraction.


Molecularly imprinted polymer-based solid-phase extraction for indomethacin


Molecularly imprinted polymer (MIP) Indomethacin (IDM) Solid-phase extraction (SPE) Water sample 



The authors thank the National Natural Science Foundation of China (NSFC, contact No. 20675054) and the Promotion Program Foundation of Sichuan University of China (No. 0082204127090) for financial support of this study. We also thank Prof. Qing-de Su, Dr. Jun Yang, and Dr. Zhong-lan Shen (University of Science and Technology of China) for their technical assistance.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Tao Yang
    • 1
  • Ya-Hui Li
    • 1
  • Shuang Wei
    • 1
  • Yuan Li
    • 1
  • Anping Deng
    • 1
    Email author
  1. 1.College of ChemistrySichuan UniversityChengduChina

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