Biosensor-based on-site explosives detection using aptamers as recognition elements
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Reliable observation, detection and characterisation of polluted soil are of major concern in regions with military activities in order to prepare efficient decontamination. Flexible on-site analysis may be facilitated by biosensor devices. With use of fibre-optic evanescent field techniques, it has been shown that immunoaffinity reactions can be used to determine explosives sensitively. Besides antibodies as molecular recognition elements, high-affinity nucleic acids (aptamers) can be employed. Aptamers are synthetically generated and highly efficient binding molecules that can be derived for any ligand, including small organic molecules like drugs, explosives or derivatives thereof. In this paper we describe the development of specific aptamers detecting the explosives molecule TNT. The aptamers are used as a sensitive capture molecule in a fibre-optic biosensor. In addition, through the biosensor measurements the aptamers could be characterised. The advantages of the aptamer biosensor include its robustness, its ability to discriminate between different explosives molecules while being insensitive to other chemical entities in natural soil and its potential to be incorporated into a portable device. Results can be obtained within minutes. The measurement is equally useful for soil that has been contaminated for a long time and for urgent hazardous spills.
KeywordsAptamer TNT In-field measurement Biosensor
We thank the German Bundesministerium für Bildung und Forschung (BMBF) and the Senate of Berlin for kindly supporting the project, Protekum GmbH for providing TNT in the beginning, as well as the Wehrwissenschaftliches Institut für Werks-, Explosiv- und Betriebsstoffe (WIWEB) for providing different analytical and soil samples.