Analytical and Bioanalytical Chemistry

, Volume 391, Issue 6, pp 2329–2338 | Cite as

Determination of illicit and medicinal drugs and their metabolites in oral fluid and preserved oral fluid by liquid chromatography–tandem mass spectrometry

  • Marta Concheiro
  • Ana de Castro
  • Óscar Quintela
  • Angelines Cruz
  • Manuel López-Rivadulla
Original Paper


An LC-MS/MS method using 0.5 ml of oral fluid was developed for the determination of morphine, codeine, 6-monoacetylmorphine, methadone, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, benzoylecgonine, cocaine, delta-9-tetrahydrocannabinol, zolpidem, zopiclone, alprazolam, clonazepam, oxazepam, nordiazepam, lorazepam, flunitrazepam, diazepam, diphenhydramine and amitriptyline. The method was fully validated in terms of linearity (the method was linear between 1–5 μg/L and 100–200 μg/L) recoveries (7.5–82.6%), within-day and between-day precisions and accuracies (CV and MRE, both <15%), limits of detection (0.5 μ g/L) and quantitation (the lowest point on the calibration curve), relative ion intensities, freeze-and-thaw stability and matrix effect. The method was applied to preserved oral fluid collected by a special commercial device, the StatSure Saliva Sampler™.


LC-MS/MS Drugs Oral fluid 



The authors thank Daniel González Aido for his technical assistance.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Marta Concheiro
    • 1
  • Ana de Castro
    • 1
  • Óscar Quintela
    • 1
  • Angelines Cruz
    • 1
  • Manuel López-Rivadulla
    • 1
  1. 1.Forensic Toxicology Service. Institute of Legal MedicineUniversity of Santiago de Compostela SpainSantiago de CompostelaSpain

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