Analytical and Bioanalytical Chemistry

, Volume 391, Issue 6, pp 2023–2034

Certification of standard reference materials containing bitter orange

  • L. C. Sander
  • K. Putzbach
  • B. C. Nelson
  • C. A. Rimmer
  • M. Bedner
  • J. Brown Thomas
  • B. J. Porter
  • L. J. Wood
  • M. M. Schantz
  • K. E. Murphy
  • K. E. Sharpless
  • S. A. Wise
  • J. H. Yen
  • P. H. Siitonen
  • R. L. Evans
  • A. Nguyen Pho
  • M. C. Roman
  • J. M. Betz
Original Paper

Abstract

A suite of three dietary supplement standard reference materials (SRMs) containing bitter orange has been developed, and the levels of five alkaloids and caffeine have been measured by multiple analytical methods. Synephrine, octopamine, tyramine, N-methyltyramine, hordenine, total alkaloids, and caffeine were determined by as many as six analytical methods, with measurements performed at the National Institute of Standards and Technology and at two collaborating laboratories. The methods offer substantial independence, with two types of extractions, two separation methods, and four detection methods. Excellent agreement was obtained among the measurements, with data reproducibility for most methods and analytes better than 5% relative standard deviation. The bitter-orange-containing dietary supplement SRMs are intended primarily for use as measurement controls and for use in the development and validation of analytical methods.

Keywords

Reference materials Natural products Organic compounds High-performance liquid chromatography Foods/beverages 

Supplementary material

216_2008_2074_MOESM1_ESM.pdf (284 kb)
ESM 1(PDF 283 KB)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • L. C. Sander
    • 1
  • K. Putzbach
    • 1
    • 2
  • B. C. Nelson
    • 1
  • C. A. Rimmer
    • 1
  • M. Bedner
    • 1
  • J. Brown Thomas
    • 1
  • B. J. Porter
    • 1
  • L. J. Wood
    • 1
  • M. M. Schantz
    • 1
  • K. E. Murphy
    • 1
  • K. E. Sharpless
    • 1
  • S. A. Wise
    • 1
  • J. H. Yen
    • 3
  • P. H. Siitonen
    • 4
  • R. L. Evans
    • 4
  • A. Nguyen Pho
    • 5
  • M. C. Roman
    • 6
    • 7
  • J. M. Betz
    • 8
  1. 1.National Institute of Standards and Technology, Chemical Science and Technology LaboratoryGaithersburgUSA
  2. 2.RCC Ltd.ItingenSwitzerland
  3. 3.National Institute of Standards and Technology, Information Technology LaboratoryGaithersburgUSA
  4. 4.National Center for Toxicological Research (NCTR), Food and Drug Administration (FDA)JeffersonUSA
  5. 5.Center for Drug Evaluation and Research (CDER), Food and Drug AdministrationSilver SpringUSA
  6. 6.ChromaDex, Inc., Research and DevelopmentClearwaterUSA
  7. 7.Tampa Bay Analytical Research, Inc.LargoUSA
  8. 8.Office of Dietary Supplements, National Institutes of HealthBethesdaUSA

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