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Analytical and Bioanalytical Chemistry

, Volume 391, Issue 2, pp 625–632 | Cite as

Characterization of interactions between polyphenolic compounds and human serum proteins by capillary electrophoresis

  • Andréa Diniz
  • Laura Escuder-Gilabert
  • Norberto P. Lopes
  • Rosa María Villanueva-Camañas
  • Salvador Sagrado
  • María José Medina-Hernández
Original Paper

Abstract

The interaction of ten natural polyphenolic compounds (chlorogenic acid, apigenin, catechin, epicatechin, flavanone, flavone, quercetin, rutin, vicenin-2 and vitexin) with human serum albumin and mixtures of human serum albumin and α1-acid glycoprotein under near physiological conditions is studied by capillary electrophoresis–frontal analysis. Furthermore, the binding of these polyphenolic compounds to total plasmatic proteins is evaluated using ultrafiltration and capillary electrophoresis. In spite of the relatively small differences in the chemical structures of the compounds studied, large differences were observed in their binding behaviours to plasmatic proteins. The hydrophobicity, the presence/absence of some functional groups, steric hindrance and spatial arrangement seem to be key factors in the affinity of natural polyphenols towards plasmatic proteins.

Keywords

Natural polyphenolic compounds Polyphenol–protein interactions Human serum albumin α1-Acid glycoprotein Whole plasma Frontal analysis Capillary electrophoresis 

Notes

Acknowledgements

The authors acknowledge the Spanish Ministry of Science and Technology (MCYT), the European Regional Development Fund (ERDF) (Project SAF2005–01435) and Generalitat Valenciana (ACOMP07–011) for the financial support. A. Diniz thanks the Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior (Capes)(BEX 1525/06–9) for the financial support. A. Diniz also thanks M.A. Martínez-Gómez for her teaching and unconditional support during all this work. L. Escuder-Gilabert is grateful to the Generalitat Valenciana for the financial support (APOSTD/2007/062).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Andréa Diniz
    • 1
    • 2
  • Laura Escuder-Gilabert
    • 3
  • Norberto P. Lopes
    • 2
  • Rosa María Villanueva-Camañas
    • 3
  • Salvador Sagrado
    • 3
  • María José Medina-Hernández
    • 3
  1. 1.Departamento de Ciências Farmacêuticas, CCSUniversidade Estadual de LondrinaLondrina-PRBrazil
  2. 2.Faculdade de Ciências FarmacêuticasUniversidade de São PauloRibeirão Preto-SPBrazil
  3. 3.Departamento de Química Analítica, Facultat de FarmaciaUniversitat de ValènciaBurjassot, ValenciaSpain

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