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Analytical and Bioanalytical Chemistry

, Volume 391, Issue 6, pp 2005–2010 | Cite as

NIES certified reference material for microcystins, hepatotoxic cyclic peptide toxins from cyanobacterial blooms in eutrophic water bodies

  • Tomoharu SanoEmail author
  • Hiroo Takagi
  • Masataka Nishikawa
  • Kunimitsu Kaya
Original Paper

Abstract

A certified reference material (CRM) for microcystins has been prepared by the National Institute for Environmental Studies (NIES). Microcystins are hepatotoxic cyclic peptides produced by cyanobacteria in eutrophic water bodies. At least seven microcystin variants were found by HPLC analysis of the NIES CRM, of which [Dha7]microcystin-RR and -LR were the major microcystins present. Because of the lack of available standards we determined the total microcystin concentration in the CRM by the MMPB method, and elucidated the structures of the main individual microcystin variants following their isolation. Analyses of NMR and MS spectra indicated that the remaining minor variants in the CRM were [D-Asp3, Dha7]microcystin-RR and -LR, and [Dha7]microcystin-YR, -ThTyrR, and -HilR. The CRM is valuable not only as a standard material for the quantitation of total microcystins but also for the identification of individual [Dha7]microcystin variants.

Keywords

Microcystins Cyanotoxin CRM Microcystis aeruginosa Water bloom 

Notes

Acknowledgements

The authors thank to Mss. Ukachi, Miura, and Nagano for much help in the preparation of the CRM, and also thank Dr. Uchida (Agilent technologies Inc., Tokyo, Japan) for LC-MS/MS experiments.

References

  1. 1.
    Kaya K, Sano T (2005) Bioactive compounds of freshwater cyanobacteria. In: Kasai F, Kaya, K, Watanabe MM (eds) Algal culture collections and the environment. Tokai University Press, HadanoGoogle Scholar
  2. 2.
    Falconer I, Bartram J, Chorus I, Kuiper-Goodman T, Utkilen H, Burch M, Codd GA (1999) Safe levels and safe practices. In: Chorus I, Bartram J (eds) Toxic cyanobacteria in water. E & FN Spon, LondonGoogle Scholar
  3. 3.
    Takagi H, Shirai M, Sano T, Kaya K (2004) J Environ Chem 14:587–596Google Scholar
  4. 4.
    Kaya K, Sano T (1999) Anal Chim Acta 386:107–112CrossRefGoogle Scholar
  5. 5.
    Fujii K, Ikai Y, Oka H, Suzuki M, Harada K-I (1997) Anal Chem 69:5146–5151CrossRefGoogle Scholar
  6. 6.
    Namikoshi M, Sun F, Choi BW, Rinehart KL, Carmichael WW, Evans WR, Beasley VR (1995) J Org Chem 60:3671–3679CrossRefGoogle Scholar
  7. 7.
    Itou Y, Ishida K, Shin SJ, Murakami M (1999) Tetrahedron 55:6871–6882CrossRefGoogle Scholar
  8. 8.
    Harada K-I, Mayumi T, Shimada T, Suzuki M, Kondo F, Watanabe MF (1993) Tetrahedron Lett 34:6091–6094CrossRefGoogle Scholar
  9. 9.
    Sano T, Takagi H, Kaya K (2004) Phytochemistry 65:2159–2162CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Tomoharu Sano
    • 1
    Email author
  • Hiroo Takagi
    • 1
  • Masataka Nishikawa
    • 1
  • Kunimitsu Kaya
    • 2
  1. 1.Laboratory of Intellectual Fundamentals for Environmental StudiesNational Institute for Environmental StudiesIbarakiJapan
  2. 2.Graduate School of Environmental StudiesTohoku UniversitySendaiJapan

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